Journal article
Oncogene N-ras mediates selective inhibition of c-fos induction by nerve growth factor and basic fibroblast growth factor in a PC12 cell line
Molecular and cellular biology, Vol.10(4), pp.1556-1563
04/1990
DOI: 10.1128/mcb.10.4.1556-1563.1990
PMCID: PMC362260
PMID: 2108319
Abstract
A cell line was generated from U7 cells (a subline of PC12 rat pheochromocytoma cells) that contains a stably integrated transforming mouse N-ras (Lys-61) gene under the control of the long terminal repeat from mouse mammary tumor virus. Such cells, designated UR61, undergo neuronal differentiation upon exposure to nanomolar concentrations of dexamethasone, as a consequence of expression of the activated N-ras gene (I. Guerrero, A. Pellicer, and D.E. Burstein, Biochem, Biophys. Res. Commun. 150:1185-1192, 1988). Exposure of UR61 cells to either nerve growth factor (NGF) or basic fibroblast growth factor (bFGF) results in a marked induction of c-fos RNA, with kinetics paralleling those of NGF- or bFGF-induced expression of c-fos RNA in PC12 cells. Dexamethasone-induced expression of activated N-ras p21 results in blocking of c-fos RNA induction by NGF or bFGF in a time-dependent manner. Activated N-ras p21-mediated inhibition of c-fos RNA induction in UR61 cells is selective for NGF and bFGF and is not due to selective degradation of c-fos RNA. Normal and transforming N-ras can trans activate the chloramphenicol acetyltransferase gene linked to mouse c-fos regulatory sequences when transient expression assays are performed. Our observations suggest that N-ras p21 selectively interacts with pathways involved in induction of c-fos expression which initiate at the receptors for NGF and bFGF.
Details
- Title: Subtitle
- Oncogene N-ras mediates selective inhibition of c-fos induction by nerve growth factor and basic fibroblast growth factor in a PC12 cell line
- Creators
- Timothy M Thomson - Department of Pathology, New York University Medical Center, New York 10016Steven H Green - Department of Pathology, New York University Medical Center, New York 10016Robert J Trotta - Department of Pathology, New York University Medical Center, New York 10016David E Burstein - Department of Pathology, New York University Medical Center, New York 10016Angel Pellicer - Department of Pathology, New York University Medical Center, New York 10016
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biology, Vol.10(4), pp.1556-1563
- DOI
- 10.1128/mcb.10.4.1556-1563.1990
- PMID
- 2108319
- PMCID
- PMC362260
- ISSN
- 0270-7306
- eISSN
- 1098-5549
- Language
- English
- Date published
- 04/1990
- Academic Unit
- Biology; Otolaryngology
- Record Identifier
- 9984217417202771
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