Journal article
One step 4x and 12x 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures
The Journal of cell biology, Vol.224(2), 202407116
12/03/2024
DOI: 10.1083/jcb.202407116
PMCID: PMC11613959
PMID: 39625433
Abstract
Super-resolution microscopy has become an indispensable tool across diverse research fields, offering unprecedented insights into biological architectures with nanometer scale resolution. Compared with traditional nanometer-scale imaging methods such as electron microscopy, super-resolution microscopy offers several advantages, including the simultaneous labeling of multiple target biomolecules with high specificity and simpler sample preparation, making it accessible to most researchers. In this study, we introduce two optimized methods of super-resolution imaging: 4-fold and 12-fold 3D-isotropic and preserved Expansion Microscopy (4x and 12x 3D-ExM). 3D-ExM is a straightforward expansion microscopy technique featuring a single-step process, providing robust and reproducible 3D isotropic expansion for both 2D and 3D cell culture models. With standard confocal microscopy, 12x 3D-ExM achieves a lateral resolution of <30 nm, enabling the visualization of nanoscale structures, including chromosomes, kinetochores, nuclear pore complexes, and Epstein-Barr virus particles. These results demonstrate that 3D-ExM provides cost-effective and user-friendly super-resolution microscopy, making it highly suitable for a wide range of cell biology research, including studies on cellular and chromatin architectures.
Details
- Title: Subtitle
- One step 4x and 12x 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures
- Creators
- Roshan X. Norman - University of Wisconsin–MadisonYu-Chia Chen - University of Wisconsin–MadisonEmma E. Recchia - University of Wisconsin–MadisonJonathan Loi - University of Wisconsin–MadisonQuincy Rosemarie - University of Wisconsin–MadisonSydney L. Lesko - University of Wisconsin–MadisonSmit Patel - University of Wisconsin–MadisonNathan Sherer - University of Wisconsin–MadisonMotoki Takaku - University of North DakotaMark E. Burkard - University of Wisconsin–MadisonAussie Suzuki - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.224(2), 202407116
- DOI
- 10.1083/jcb.202407116
- PMID
- 39625433
- PMCID
- PMC11613959
- NLM abbreviation
- J Cell Biol
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Publisher
- Rockefeller Univ Press
- Number of pages
- 25
- Grant note
- UND COBRE pilot genomics awards Wisconsin Partnership Program, the University of Wisconsin-Madison Office of the Vice Chancellor for Research U54AI170660; R01GM131068; R01CA234904; R01AI110221; P01CA022443; R01GM148729 / CRNA grant University of Wisconsin-Madison SMPH Wisconsin Alumni Research Foundation R35GM147525 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA UW Carbone Cancer Center McArdle Laboratory for Cancer Research
- Language
- English
- Date published
- 12/03/2024
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984757685402771
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