Ontogeny and distribution of the murine B cell Fc gamma RII

Teresa M Foy; Richard G Lynch; Thomas J Waldschmidt

doi:10.4049/jimmunol.149.5.1516

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Ontogeny and distribution of the murine B cell Fc gamma RII

Journal article

Peer reviewed

Ontogeny and distribution of the murine B cell Fc gamma RII

Teresa M Foy, Richard G Lynch and Thomas J Waldschmidt

The Journal of immunology (1950), Vol.149(5), pp.1516-1523

09/01/1992

DOI: 10.4049/jimmunol.149.5.1516

PMID: 1387140

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Abstract

The distribution and expression of the IgG FcRII (Fc gamma RII) on normal murine B cells was examined. Using multicolor flow cytometry, spleens from neonatal mice of increasing age and adult bone marrow were analyzed for expression of the Fc gamma RII. In addition, B cells from peripheral lymphoid organs, as well as panel of B cell tumors, were tested. The results demonstrate that the Fc gamma RII is expressed on all pre-B cells and immature B cells in the neonatal spleen and adult bone marrow, on all mature B cells in peripheral lymphoid organs, and on switched B cells in Peyer's patches. Furthermore, the Fc gamma RII was found to be present on B cell tumors representative of all stages of B cell maturation and differentiation. Taken together, the results indicate that Fc gamma RII is expressed during the entire lifetime of the B cell. In addition, examination of spleen cells from neonatal mice revealed a large number of pre-B cells, phenotypically defined as B220+, IgM-. These pre-B cells were present at birth, peaked in number between 2 and 3 wk of age, and became a minor population by day 30. Further phenotypic analysis of these cells demonstrated the expression of the BLA-1 and BP-1 Ag, and the lack of T cell and NK cell markers, thus confirming their assignment to the B cell lineage. Finally, the Fc gamma RII present on these pre-B cells was shown to be functional, by virtue of its ability to bind aggregated IgG.

Flow Cytometry

Antigens, Differentiation - analysis

Receptors, Fc - analysis

Immunoglobulin M - analysis

Antigens, Differentiation - physiology

Receptors, IgG

Hematopoietic Stem Cells - immunology

Animals

B-Lymphocytes - immunology

B-Lymphocyte Subsets - immunology

Mice

Mice, Inbred BALB C

Receptors, Fc - physiology

Immunoglobulin G - metabolism

Details

Title: Subtitle: Ontogeny and distribution of the murine B cell Fc gamma RII
Creators: Teresa M Foy - Department of Pathology, University of Iowa College of Medicine, Iowa City 52242
Richard G Lynch
Thomas J Waldschmidt
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.149(5), pp.1516-1523
DOI: 10.4049/jimmunol.149.5.1516
PMID: 1387140
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: CA 49228 / NCI NIH HHS
Language: English
Date published: 09/01/1992
Academic Unit: Pathology
Record Identifier: 9984047757302771

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