Journal article
Ontogeny of renal beta adrenoceptors in the sheep
Pediatric nephrology (Berlin, West), Vol.4(6), pp.635-639
11/01/1990
DOI: 10.1007/BF00858641
PMID: 1982416
Abstract
The renal vasodilatory response to beta-2 adrenoceptor stimulation is greater in fetal than adult sheep. Since this phenomenon could not be explained by differences in cAMP-mediated events, we examined the ontogeny of renal beta adrenoceptor subtypes by radioligand binding using the beta adrenergic antagonist125I-pindolol. The specific binding of125I-pindolol was saturable, reversible, and stereoselective. Competition studies using the selective beta-1 and beta-2 adrenergic antagonists (ICI 89406 and ICI 118551, respectively) revealed two beta adrenoceptor subtypes in adult kidneys but only the beta-2 subtype in fetal kidneys. Preincubation of renal cortical membranes with either the beta-1 or the beta-2 adrenergic antagonist prior to competition studies in adult kidneys decreased specific binding and resulted in the detection of only one beta adrenoceptor subtype. Preincubation of fetal kidneys with the beta-1 adrenergic antagonist did not affect binding characteristics; preincubation of fetal kidneys with the beta-2 adrenergic antagonist markedly decreased specific binding from 64±2% (n=4) to 29±2% (n=3) (P<0.05). Analysis of Rosenthal plots revealed similar beta adrenoceptor densities and affinities between fetal and adult kidneys. Since the beta-2 adrenoceptor subtype is mainly found in the fetal kidney, while both adrenoceptor subtypes are found in adult kidneys, the density of renal beta-2 adrenoceptors is greater in fetal than adult kidneys. It is possible that the greater renal vasodilatory effect of beta-2 adrenergic agonist in fetal kidneys is due to increased density of beta-2 adrenoceptors.
Details
- Title: Subtitle
- Ontogeny of renal beta adrenoceptors in the sheep
- Creators
- Christian C. Felder - National Institute of Mental HealthMarlene M. Piccio - Georgetown UniversityAnthony M. McKelvey - Georgetown UniversityKenneth T. Nakamura - University of Hawaiʻi at MānoaJean E. Robillard - University of IowaPedro A. Jose - Georgetown University Medical Center
- Resource Type
- Journal article
- Publication Details
- Pediatric nephrology (Berlin, West), Vol.4(6), pp.635-639
- DOI
- 10.1007/BF00858641
- PMID
- 1982416
- ISSN
- 0931-041X
- eISSN
- 1432-198X
- Number of pages
- 5
- Language
- English
- Date published
- 11/01/1990
- Academic Unit
- Molecular Physiology and Biophysics; Nephrology, Dialysis and Transplantation; Stead Family Department of Pediatrics; Medicine Administration
- Record Identifier
- 9984773399102771
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