Ontogeny of vascular growth factors in perinatal sheep myocardium

Timothy L van Natta; J Carter Ralphe; Christopher E Mascio; Kurt A Bedell; Thomas D Scholz; Jeffrey L Segar

doi:10.1016/j.jsgi.2004.05.010

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Journal article

Ontogeny of vascular growth factors in perinatal sheep myocardium

Timothy L van Natta, J Carter Ralphe, Christopher E Mascio, Kurt A Bedell, Thomas D Scholz and Jeffrey L Segar

Journal of the Society for Gynecologic Investigation, Vol.11(8), pp.503-510

12/2004

DOI: 10.1016/j.jsgi.2004.05.010

PMID: 15582494

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Abstract

To examine developmental changes in myocardial gene expression of previously identified regulators of vascular growth. Ovine left (LV) and right ventricle (RV) samples were obtained at four time points: 95 days' and 140 days' gestation (term = 145 days) and 7 days and 8 weeks postnatally. mRNA and protein levels of vascular endothelial growth factor (VEGF), its respective receptors (Flk-1 and Flt-1), basic fibroblast growth factor (bFGF), transforming growth factor-beta1 (TGF-beta1), and endothelial nitric oxide synthase (eNOS) were measured at these different time points. RV but not LV VEGF mRNA levels decreased postnatally, although VEGF protein expression remained unchanged after birth. Flt-1 mRNA expression was divergent between ventricles, although the protein expression pattern was similar in RV and LV, decreasing with maturation. RV and LV Flk-1 mRNA decreased between 95 days and 140 days, remaining stable thereafter, while protein levels only decreased after birth. bFGF protein levels were highest in the LV at 140 days, and decreased after birth but remained unchanged in the RV throughout the period examined. TGF-beta1 and eNOS levels were highest early in gestation, decreasing with maturation in both ventricles. Developmentally regulated ventricle-specific expression of VEGF, Flt-1, Flk-1, TGF-beta1, bFGF, and eNOS was demonstrated in the ovine myocardium. These findings suggest these proteins may participate in coronary vascular remodeling during the perinatal period and underscore the importance of studying the relationships among transcription factors, target genes, and anatomic/physiologic changes in the whole animal.

Myocardium - chemistry

RNA, Messenger - analysis

Male

Nitric Oxide Synthase - genetics

Vascular Endothelial Growth Factor A - genetics

Vascular Endothelial Growth Factor Receptor-2 - genetics

Growth Substances - genetics

Transforming Growth Factor beta - analysis

Fibroblast Growth Factor 2 - analysis

Gene Expression Regulation, Developmental

Aging

Female

Vascular Endothelial Growth Factor Receptor-1 - genetics

Animals, Newborn

Nitric Oxide Synthase - analysis

Transforming Growth Factor beta1

Vascular Endothelial Growth Factor Receptor-2 - analysis

Nitric Oxide Synthase Type III

Vascular Endothelial Growth Factor A - analysis

Pregnancy

Heart Ventricles - chemistry

Animals

Transforming Growth Factor beta - genetics

Fibroblast Growth Factor 2 - genetics

Vascular Endothelial Growth Factor Receptor-1 - analysis

Growth Substances - analysis

Sheep

Details

Title: Subtitle: Ontogeny of vascular growth factors in perinatal sheep myocardium
Creators: Timothy L van Natta - Department of Surgery, University of Iowa, Iowa City, Iowa 52242, USA
J Carter Ralphe
Christopher E Mascio
Kurt A Bedell
Thomas D Scholz
Jeffrey L Segar
Resource Type: Journal article
Publication Details: Journal of the Society for Gynecologic Investigation, Vol.11(8), pp.503-510
DOI: 10.1016/j.jsgi.2004.05.010
PMID: 15582494
ISSN: 1071-5576
eISSN: 1556-7117
Grant note: K02-HL-04495 / NHLBI NIH HHS T32 HL007413 / NHLBI NIH HHS R01-HL-56633 / NHLBI NIH HHS R01-HL-64770 / NHLBI NIH HHS
Language: English
Date published: 12/2004
Academic Unit: Microbiology and Immunology; Cardiology; Stead Family Department of Pediatrics; Fraternal Order of Eagles Diabetes Research Center; Child and Community Health
Record Identifier: 9984093370202771

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