Oocyte Host-Transfer and Maternal mRNA Depletion Experiments in Xenopus

Douglas W Houston

doi:10.1101/pdb.prot096982

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Journal article

Oocyte Host-Transfer and Maternal mRNA Depletion Experiments in Xenopus

Douglas W Houston

Cold Spring Harbor protocols, Vol.2018(10), pp.803-809

10/01/2018

DOI: 10.1101/pdb.prot096982

PMCID: PMC6538067

PMID: 29321286

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Abstract

This protocol details the oocyte host-transfer method in , using transplantation by intraperitoneal injection. This approach is suitable for the overexpression of mRNAs and for the use of antisense oligonucleotides to deplete maternal mRNAs, which are not replaced until zygotic genome activation in the mid-blastula transition. oocyte host-transfer can also be used for highly efficient mutagenesis in the F generation by prefertilization injection of genome editing reagents.

Oocytes - cytology

Animals

Fertilization in Vitro

In Vitro Oocyte Maturation Techniques - methods

Oocytes - metabolism

Xenopus laevis - metabolism

Humans

Cells, Cultured

Injections, Intraperitoneal

Female

RNA, Messenger - metabolism

Details

Title: Subtitle: Oocyte Host-Transfer and Maternal mRNA Depletion Experiments in Xenopus
Creators: Douglas W Houston - Department of Biology, The University of Iowa, Iowa City, Iowa 52242-1324 douglas-houston@uiowa.edu
Resource Type: Journal article
Publication Details: Cold Spring Harbor protocols, Vol.2018(10), pp.803-809
DOI: 10.1101/pdb.prot096982
PMID: 29321286
PMCID: PMC6538067
NLM abbreviation: Cold Spring Harb Protoc
ISSN: 1559-6095
eISSN: 1559-6095
Publisher: United States
Grant note: R01 GM083999 / NIGMS NIH HHS
Language: English
Date published: 10/01/2018
Academic Unit: Biology
Record Identifier: 9983991938102771

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