Journal article
Ophthalmopathology in rats with MBP-induced experimental autoimmune encephalomyelitis
Graefe's archive for clinical and experimental ophthalmology, Vol.249(7), pp.1009-1020
07/2011
DOI: 10.1007/s00417-011-1633-9
PMID: 21344308
Abstract
Multiple studies indicate that T-cells play a major role in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), the animal model of multiple sclerosis, but recently an involvement of antibodies has also been discussed. The aim of our study was to examine the effects of myelin basic protein (MBP) immunization on survival of neurons, alteration of antibody reactivity, and microglia in the retinal ganglion cell layer.EAE was induced in rats by immunization with MBP. Intraocular pressure (IOP) measurements and funduscopies were performed regularly. Neuron cell density was evaluated on cresyl-stained retinal flatmounts. IgG antibody deposition and activated microglia were detected in retina and optic nerve sections via immunohistology. The intensity of autoreactive IgG antibodies was quantified in successive serum samples via tissue arrays.Significant loss of neurons was detected 6 weeks after immunization (p < 0.05). At the same time, IgG antibody deposits accumulated in the retina and the optic nerve of EAE animals and a significant microglia turn-over to activation was observed. The level of IgG antibody reactivity against retina and optic nerve tissue continuously increased (p < 0.05). While clinical parameters indicated typical EAE progression, we observed no changes in IOP (p > 0.9) or abnormalities in fundi.Immunization with MBP not only causes neuron loss in the retinal ganglion cell layer, but also triggers antibody reactivity against ocular tissue. Possibly some of these antibodies are involved in the induction of neuronal apoptosis. This study suggests that, apart from T-cell mediation, alteration of antibody reactivity and activated microglia do also influence the ocular pathomechanisms in the EAE model.
Details
- Title: Subtitle
- Ophthalmopathology in rats with MBP-induced experimental autoimmune encephalomyelitis
- Creators
- Oliver Gramlich - Experimental Ophthalmology, Department of Ophthalmology, University Medical Center Johannes Gutenberg University Mainz Langenbeckstraße 1 55101 Mainz GermanyStephanie Joachim - Center for Vision Science Ruhr University Eye Hospital In der Schornau 23-25 44892 Bochum GermanyPhilip Gottschling - Experimental Ophthalmology, Department of Ophthalmology, University Medical Center Johannes Gutenberg University Mainz Langenbeckstraße 1 55101 Mainz GermanyPanagoitis Laspas - Experimental Ophthalmology, Department of Ophthalmology, University Medical Center Johannes Gutenberg University Mainz Langenbeckstraße 1 55101 Mainz GermanyClemens Cuny - Experimental Ophthalmology, Department of Ophthalmology, University Medical Center Johannes Gutenberg University Mainz Langenbeckstraße 1 55101 Mainz GermanyNorbert Pfeiffer - Experimental Ophthalmology, Department of Ophthalmology, University Medical Center Johannes Gutenberg University Mainz Langenbeckstraße 1 55101 Mainz GermanyFranz Grus - Experimental Ophthalmology, Department of Ophthalmology, University Medical Center Johannes Gutenberg University Mainz Langenbeckstraße 1 55101 Mainz Germany
- Resource Type
- Journal article
- Publication Details
- Graefe's archive for clinical and experimental ophthalmology, Vol.249(7), pp.1009-1020
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- DOI
- 10.1007/s00417-011-1633-9
- PMID
- 21344308
- ISSN
- 0721-832X
- eISSN
- 1435-702X
- Language
- English
- Date published
- 07/2011
- Academic Unit
- Iowa Neuroscience Institute; Neuroscience and Pharmacology; Ophthalmology and Visual Sciences
- Record Identifier
- 9984070678102771
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