Journal article
Opiate regulation of IL-1β and TNF-α in cultured human articular chondrocytes
Biochemical and biophysical research communications, Vol.333(4), pp.1295-1299
2005
DOI: 10.1016/j.bbrc.2005.06.035
PMID: 15979578
Abstract
In order to investigate if β-endorphins anti-inflammatory effect in cartilage-damaging states is mediated via tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), we examined its influence on these two cytokines in vitro. Human articular chondrocytes were obtained from patients undergoing total knee arthroplasty and stimulated with β-endorphin (60–6000
ng/ml). Protein levels of TNF-α and IL-1β were measured by ELISA in supernatants from articular chondrocyte cultures. β-Endorphin significantly increased the levels of IL-1β for all concentrations used after 15
min incubation, and when stimulated with 600 and 6000
ng/ml after 24
h incubation. The opioid-induced increase in IL-1β was blocked by naltrexone in the group tested. TNF-α expression was also significantly stimulated by 60 and 600
ng/ml β-endorphin after 15
min, an effect blocked by naltrexone in the group tested. These findings indicate that the mechanism of β-endorphins anti-inflammatory influence in cartilage-damaging states is not apparently mediated via these two cytokines modulation.
Details
- Title: Subtitle
- Opiate regulation of IL-1β and TNF-α in cultured human articular chondrocytes
- Creators
- Nenad Andjelkov - Department of Orthopaedics, University Hospital of Northern-Norway, 9038 Tromsø, NorwayJan Elvenes - Department of Orthopaedics, University Hospital of Northern-Norway, 9038 Tromsø, NorwayJames Martin - Department of Orthopaedic Surgery, The University of Iowa, Iowa City, IA 52242, USAOddmund Johansen - Department of Orthopaedics, University Hospital of Northern-Norway, 9038 Tromsø, Norway
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.333(4), pp.1295-1299
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.bbrc.2005.06.035
- PMID
- 15979578
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 2005
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation
- Record Identifier
- 9984040337002771
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