Journal article
Opioid system blockade decreases collagenase activity and improves liver injury in a rat model of cholestasis
Journal of gastroenterology and hepatology, Vol.22(3), pp.406-413
Accepted for publication 23 October 2005.
03/2007
DOI: 10.1111/j.1440-1746.2006.04260.x
PMID: 17295775
Abstract
Background: Following bile duct ligation (BDL) endogenous opioids accumulate in plasma and play a role in the pathophysiology and manifestation of cholestasis. Evidence of centrally mediated induction of liver injury by exogenous opioid agonist administration, prompts the question of whether opioid receptor blockade by naltrexone can affect cholestasis-induced liver injury. Methods: Cholestasis was induced by BDL and cholestatic and sham-operated rats received either naltrexone or saline for 7 consecutive days. On the 7th day, liver samples were collected for determining matrix metalloproteinase-2 (MMP-2) activity, S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) content and blood samples were obtained for measuring plasma nitrite/nitrate and liver enzyme activities. Results: Naltrexone-treated BDL animals had a significant reduction in plasma enzyme activity and nitrite/nitrate level. Liver SAM: SAH ratio and SAM level improved by naltrexone treatment in cholestatic animals compared to saline-treated BDL ones. Naltrexone treatment in BDL rats led to a decrease in the level of liver MMP-2 activity, which had already increased during cholestasis. Conclusion: Opioid receptor blockade improved the degree of liver injury in cholestasis, as assessed by plasma enzyme and liver MMP-2 activities. The beneficial effect of naltrexone may be due to its ability to increase liver SAM level and restore the SAM: SAH ratio. © 2007 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.
Details
- Title: Subtitle
- Opioid system blockade decreases collagenase activity and improves liver injury in a rat model of cholestasis
- Creators
- Samira Kiani - Tehran University of Medical SciencesMohammad R Ebrahimkhani - Departments of Pharmacology andAhmad Shariftabrizi - Departments of Pharmacology andBehzad Doratotaj - Departments of Pharmacology andSeyedmehdi Payabvash - Departments of Pharmacology andKiarash Riazi - Departments of Pharmacology andMehdi Dehghani - Departments of Pharmacology andHooman Honar - Departments of Pharmacology andAlaleh Karoon - Departments of Pharmacology andMassoud Amanlou - Tehran University of Medical SciencesSeyed M Tavangar - Pathology, School of Medicine andAhmad R Dehpour - Departments of Pharmacology and
- Resource Type
- Journal article
- Publication Details
- Journal of gastroenterology and hepatology, Vol.22(3), pp.406-413
- Edition
- Accepted for publication 23 October 2005.
- Publisher
- Blackwell Publishing Asia
- DOI
- 10.1111/j.1440-1746.2006.04260.x
- PMID
- 17295775
- ISSN
- 0815-9319
- eISSN
- 1440-1746
- Number of pages
- 8
- Language
- English
- Date published
- 03/2007
- Academic Unit
- Radiology
- Record Identifier
- 9984318722102771
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