Optical coherence tomography and molecular analysis of sudden acquired retinal degeneration syndrome (SARDS) eyes suggests the immune‐mediated nature of retinal damage

Sinisa D Grozdanic; Tatjana Lazic; Helga Kecova; Kabhilan Mohan; Markus H Kuehn

doi:10.1111/vop.12597

Back

Optical coherence tomography and molecular analysis of sudden acquired retinal degeneration syndrome (SARDS) eyes suggests the immune‐mediated nature of retinal damage

Journal article

Open access

Peer reviewed

Optical coherence tomography and molecular analysis of sudden acquired retinal degeneration syndrome (SARDS) eyes suggests the immune‐mediated nature of retinal damage

Sinisa D Grozdanic, Tatjana Lazic, Helga Kecova, Kabhilan Mohan and Markus H Kuehn

Veterinary ophthalmology, Vol.22(3), pp.305-327

05/2019

DOI: 10.1111/vop.12597

PMCID: PMC6563498

PMID: 30109754

Files and links (1)

url

https://doi.org/10.1111/vop.12597View

Published (Version of record) Open Access

Abstract

Objective To perform detailed analysis of retinal changes in dogs with SARDS using optical coherence tomography (OCT), funduscopy, and molecular analysis. Animals Subjects were 29 dogs from 12 US states and Canada diagnosed with SARDS by 8 ophthalmologists. An additional 7 eyes from 5 deceased SARDS dogs were used for molecular and histological analysis. Procedures Dogs were evaluated using chromatic pupil light reflex testing (cPLR), and electroretinography (ERG); subjects underwent complete ophthalmic examination, including funduscopy, retinal photography, and OCT, in addition to complete laboratory analysis, blood pressure evaluation, abdominal and thoracic radiographs, and computerized tomography (CT) imaging to assess possible systemic abnormalities. Histology and immunohistochemistry analysis was performed in 2 SARDS eyes. Microarray analysis was performed in 5 SARDS retinas. Results Thirty‐eight percent of patients had <1‐mm wide retinal detachments (RD) on OCT analysis, which could not be detected by funduscopy or retinal photographs. Systemic hypertension did not seem to be a contributing factor (RD 22.2%; ND 20%, Odds ratio = 1.1). No dogs showed neoplastic changes by thoracic or abdominal radiography, or CT imaging. There was no statistically significant difference in age (RD 7.9 ± 1.9 years (mean ± SD); ND 7.6 ± 1.7 years, p = 0.69) or duration of blindness prior to presentation (RD 18 ± 7 days (mean±SD); ND 21 ± 12 days, p = 0.28). Microarray and histology analysis of SARDS eyes revealed molecular changes suggestive of immune‐mediated damage. Conclusions Observed histological, molecular, and OCT changes are highly suggestive of immune‐mediated damage in SARDS eyes.

canine

detachment

immune

microarray

optical coherence tomography

retina

sudden acquired retinal degeneration syndrome

Details

Title: Subtitle: Optical coherence tomography and molecular analysis of sudden acquired retinal degeneration syndrome (SARDS) eyes suggests the immune‐mediated nature of retinal damage
Creators: Sinisa D Grozdanic - Iowa State University
Tatjana Lazic - Iowa State University
Helga Kecova - Iowa State University
Kabhilan Mohan - Iowa State University
Markus H Kuehn - University of Iowa
Resource Type: Journal article
Publication Details: Veterinary ophthalmology, Vol.22(3), pp.305-327
DOI: 10.1111/vop.12597
PMID: 30109754
PMCID: PMC6563498
NLM abbreviation: Vet Ophthalmol
ISSN: 1463-5216
eISSN: 1463-5224
Number of pages: 23
Grant note: U.S. Department of Veterans Affairs (C3919R) Iowa State University Biotechnology Fund Sophie's SARDS Research Fund
Language: English
Date published: 05/2019
Academic Unit: Ophthalmology and Visual Sciences
Record Identifier: 9984182754302771

Metrics

90 Record Views

25 Times Cited - Web of Science