Journal article
Optimal methods for collecting and storing vaginal specimens for prostate-specific antigen testing in research studies
Contraception (Stoneham), Vol.87(6), pp.830-835
06/01/2013
DOI: 10.1016/j.contraception.2012.09.033
PMCID: PMC4452941
PMID: 23121826
Abstract
Background: Prostate-specific antigen (PSA) detected in vaginal fluid can be used in studies of HIV/sexually transmitted infection (STI) and pregnancy prevention as an alternative to relying on participant reports of exposure to semen. Optimal methods for collecting and storing specimens for this testing have not been determined.
Study Design: We conducted a controlled, in vitro experiment of 550 specimens spiked with semen to determine the effects of swab type (five types), storage conditions of the swabs (room temperature with or without desiccant or at -80 degrees C without desiccant) and time from collection to testing (seven intervals over the course of 12 months) on the identification of PSA. We performed factorial analysis of variance to identify factors influencing PSA detection.
Results: Concentrations of PSA detected in the swabs declined with time of storage over the 1-year experiment (p<.01). The 1-mL, rayon-tipped swab stored immediately at -80 degrees C following collection performed best.
Conclusions: If immediate testing or freezer storage is not feasible, investigators should use a swab with 1-mL capacity with processing and testing as soon as possible after specimen collection. Published by Elsevier Inc.
Details
- Title: Subtitle
- Optimal methods for collecting and storing vaginal specimens for prostate-specific antigen testing in research studies
- Creators
- Maria F. Gallo - National Center for Chronic Disease Prevention and Health PromotionMargaret C. Snead - National Center for Chronic Disease Prevention and Health PromotionCarolyn M. Black - National Center for Emerging and Zoonotic Infectious DiseasesTeresa M. Brown - National Center for Emerging and Zoonotic Infectious DiseasesAthena P. Kourtis - Centers for Disease Control and PreventionDenise J. Jamieson - National Center for Chronic Disease Prevention and Health PromotionMarion Carter - National Center for Chronic Disease Prevention and Health PromotionAna Penman-Aguilar - National Center for Chronic Disease Prevention and Health PromotionMaurizio Macaluso - Cincinnati Children's Hospital Medical Center
- Resource Type
- Journal article
- Publication Details
- Contraception (Stoneham), Vol.87(6), pp.830-835
- Publisher
- Elsevier
- DOI
- 10.1016/j.contraception.2012.09.033
- PMID
- 23121826
- PMCID
- PMC4452941
- ISSN
- 0010-7824
- eISSN
- 1879-0518
- Number of pages
- 6
- Grant note
- CC999999 / Intramural CDC HHS
- Language
- English
- Date published
- 06/01/2013
- Academic Unit
- Obstetrics and Gynecology; VPMA - Administration
- Record Identifier
- 9984446281802771
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