Journal article
Optimization of biomass, vitamins, and carotenoid yield on light energy in a flat-panel reactor using the A-stat technique
Biotechnology and bioengineering, Vol.89(2), pp.233-242
01/20/2005
DOI: 10.1002/bit.20346
PMID: 15593095
Abstract
Acceleration-stat (A-stat) cultivations in which the dilution rate is continuously changed at a constant acceleration rate, leading to different average light intensities inside the photobioreactor, can supply more information and reduce experimental time compared with chemostat cultivations. The A-stat was used to optimize the biomass and product yield of continuous cultures of the microalgae D. tertiolecta in a flat-panel reactor. In this study, four different accelerations were studied, a pseudo steady state was maintained at acceleration rates of 0.00016 and 0.00029 h-2 and results were similar to those of the chemostat. An increase in the acceleration rate led to an increase in the deviation between results obtained in the A-stat and in the chemostats. We concluded that it is advantageous to use the A-stat instead of chemostats to determine culture characteristics and optimize a specific photobioreactor. The effect of average light intensity inside the photobioreactor on the production of vitamins C and E, lutein, and β-carotene was studied using the A-stat. The highest concentrations of these products were 3.48 ± 0.46, 0.33 ± 0.06, 5.65 ± 0.24, and 2.36 ± 0.38 mg g-1 respectively. These results were obtained at different average light intensities, showing the importance of optimizing each product on light intensity. © 2004 Wiley Periodicals, Inc.
Details
- Title: Subtitle
- Optimization of biomass, vitamins, and carotenoid yield on light energy in a flat-panel reactor using the A-stat technique
- Creators
- Maria J. BarbosaJan Willem Zijffers - Wageningen University & ResearchAdrian Nisworo - Food & NutritionWoulter Vaes - Food & NutritionJan van Schoonhoven - Food & NutritionRené H. Wijffels - Wageningen University & Research
- Resource Type
- Journal article
- Publication Details
- Biotechnology and bioengineering, Vol.89(2), pp.233-242
- DOI
- 10.1002/bit.20346
- PMID
- 15593095
- NLM abbreviation
- Biotechnol Bioeng
- ISSN
- 0006-3592
- eISSN
- 1097-0290
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- Number of pages
- 10
- Language
- English
- Date published
- 01/20/2005
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984397923902771
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