Journal article
Optimizing Donor Cellular Dissociation and Subretinal Injection Parameters for Stem Cell‐Based Treatments
Stem Cells Translational Medicine, Vol.8(8), pp.797-809
2019
DOI: 10.1002/sctm.18-0210
PMCID: PMC6646699
PMID: 31004408
Abstract
Subretinal delivery of stem cell‐derived retinal cells as a strategy to treat retinal degenerative blindness holds great promise. Currently, two clinical trials are underway in which human fetal retinal progenitor cells (RPCs) are being delivered to patients by intravitreal or subretinal injection to preserve or restore vision, respectively. With the advent of the induced pluripotent stem cell (iPSC), and in turn three‐dimensional derivation of retinal tissue, it is now possible to generate autologous RPCs for cell replacement. The purpose of this study was to evaluate the effect of commonly used cell isolation and surgical manipulation strategies on donor cell viability. iPSC‐RPCs were subjected to various conditions, including different dissociation and isolation methods, injection cannula sizes, and preinjection storage temperatures and times. The effects of commonly used surgical techniques on both host and donor cell viability were evaluated in Yucatan mini‐pigs ( n = 61 eyes). We found a significant increase in cell viability when papain was used for RPC isolation. In addition, a significant decrease in cell viability was detected when using the 41G cannula compared with 31G and at storage times of 4 hours compared with 30 minutes. Although 96.4% of all eyes demonstrated spontaneous retinal reattachment following injection, retinal pigment epithelium (RPE) abnormalities were seen more frequently in eyes receiving injections via a 31G cannula; interestingly, eyes that received cell suspensions were relatively protected against such RPE changes. These findings indicate that optimization of donor cell isolation and delivery parameters should be considered when developing a subretinal cell replacement strategy. stem cells translational medicine 2019;8:797&809
Details
- Title: Subtitle
- Optimizing Donor Cellular Dissociation and Subretinal Injection Parameters for Stem Cell‐Based Treatments
- Creators
- Brittni A Scruggs - University of IowaChunhua Jiao - University of IowaCathryn M Cranston - University of IowaEmily Kaalberg - University of IowaKai Wang - College of Public Health, University of IowaStephen R Russell - University of IowaLuke A Wiley - University of IowaRobert F Mullins - University of IowaEdwin M Stone - University of IowaBudd A Tucker - University of IowaElliott H Sohn - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Stem Cells Translational Medicine, Vol.8(8), pp.797-809
- DOI
- 10.1002/sctm.18-0210
- PMID
- 31004408
- PMCID
- PMC6646699
- NLM abbreviation
- Stem Cells Transl Med
- ISSN
- 2157-6564
- eISSN
- 2157-6580
- Publisher
- John Wiley & Sons, Inc; Hoboken, USA
- Grant note
- NEI R01‐EY026547; P30 EY025580 / University of Iowa Institute for Vision Research Endowment Lidsky/Zawadzki/McGuinn/Cohen Families Hope for Vision VitreoRetinal Surgery Foundation (VRSF) Howard F. Ruby Endowment for Human Retinal Engineering
- Alternative title
- Conditions for Retinal Stem Cell‐Based Therapies
- Language
- English
- Date published
- 2019
- Academic Unit
- Iowa Neuroscience Institute; Biostatistics; Ophthalmology and Visual Sciences
- Record Identifier
- 9983979950002771
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