Journal article
Optimum HPLC parameters for simultaneous determination of Robinin and Kaempferol
Pharmaceutical chemistry journal, Vol.46(1), pp.64-67
04/01/2012
DOI: 10.1007/s11094-012-0735-y
Abstract
An HPLC method for simultaneous quantitative determination of robinin and its aglycone kaempferol in MCF-7 human breast carcinoma cell line was developed. Robinin and kaempferol were separated using a Platinum EPS C-18 column (4.6 x 250 nm, 5 mu m particle size). The detector was set at 380 nm. The mobile phase consisted of solution A [5 % MeOH in phosphate buffer (0.01 M, pH 2.0)] and solution B (THF:-PrOH:MeOH:H2O, 150:200:67.5:32.5) in a 60:40 ratio (A:B). Calibration curves for both compounds were linear ( (2) = 0.9997, (2) = 1) in the concentration range 25 - 1000 ng/mL. The intra- and interday variation coefficients were 1.31 - 8.92 %. The mean recoveries were 97.3 - 104.6 %. Robinin, a flavonoid glycoside isolated from leaves and flowers of , decreases residual nitrogen, creatine, and urea in blood. The finished tablet dosage form prepared from it, flaronin, is used to treat uremia caused by chronic kidney failure.
Details
- Title: Subtitle
- Optimum HPLC parameters for simultaneous determination of Robinin and Kaempferol
- Creators
- L. K. Tsiklauri - Agladze Inorganic Chemistry and ElectrochemistryG. An - University at Buffalo, State University of New YorkM. D. Alania - Agladze Inorganic Chemistry and ElectrochemistryE. P. Kemertelidze - Agladze Inorganic Chemistry and ElectrochemistryM. E. Morris - University at Buffalo, State University of New York
- Resource Type
- Journal article
- Publication Details
- Pharmaceutical chemistry journal, Vol.46(1), pp.64-67
- Publisher
- Springer Nature
- DOI
- 10.1007/s11094-012-0735-y
- ISSN
- 0091-150X
- eISSN
- 1573-9031
- Number of pages
- 4
- Grant note
- Fullbright Foundation
- Language
- English
- Date published
- 04/01/2012
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984366033702771
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