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Optogenetic inhibition of cocaine seeking in rats
Journal article   Open access   Peer reviewed

Optogenetic inhibition of cocaine seeking in rats

Michael T Stefanik, Khaled Moussawi, Yonatan M Kupchik, Kyle C Smith, Rachel L Miller, Mary L Huff, Karl Deisseroth, Peter W Kalivas and Ryan T LaLumiere
Addiction biology, Vol.18(1), pp.50-53
01/2013
DOI: 10.1111/j.1369-1600.2012.00479.x
PMCID: PMC3578202
PMID: 22823160
url
https://doi.org/10.1111/j.1369-1600.2012.00479.xView
Published (Version of record) Open Access

Abstract

Inhibitory optogenetics was used to examine the roles of the prelimbic cortex (PL), the nucleus accumbens core (NAcore) and the PL projections to the NAcore in the reinstatement of cocaine seeking. Rats were microinjected into the PL or NAcore with an adeno‐associated virus containing halorhodopsin or archaerhodopsin. After 12 days of cocaine self‐administration, followed by extinction training, animals underwent reinstatement testing along with the presence/absence of optically induced inhibition via laser light. Bilateral optical inhibition of the PL, NAcore or the PL fibers in the NAcore inhibited the reinstatement of cocaine seeking.
nucleus accumbens core halorhodopsin prelimbic cortex eNpHR3.0 reinstatement ArchT

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