Oral administration of CpG-ODNs suppresses antigen-induced asthma in mice

K KITAGAKI; T. R BUSINGA; J. N KLINE

doi:10.1111/j.1365-2249.2005.03003.x

Back

Oral administration of CpG-ODNs suppresses antigen-induced asthma in mice

Journal article

Open access

Peer reviewed

Oral administration of CpG-ODNs suppresses antigen-induced asthma in mice

K KITAGAKI, T. R BUSINGA and J. N KLINE

Clinical and experimental immunology, Vol.143(2), pp.249-259

2006

DOI: 10.1111/j.1365-2249.2005.03003.x

PMCID: PMC1809575

PMID: 16412048

Files and links (1)

url

https://doi.org/10.1111/j.1365-2249.2005.03003.xView

Published (Version of record) Open Access

Abstract

Oligodeoxynucleotides containing CpG motifs (CpG-ODNs) can protect against eosinophilic airway inflammation in asthma. Previously we have found that parenteral or mucosal administration of CpG-ODNs is effective in preventing (as well as reversing established) disease. In this study, we examined the effect of oral CpG-ODNs on the development of immune tolerance. Using an ovalbumin (OVA)-induced murine model of asthma, we found that CpG-ODNs, administered orally around the time of sensitization, prevented eosinophilic airway inflammation in a dose-dependent manner. Although oral co-administration of CpG-ODNs with OVA (known to induce tolerance) did not significantly change the inhibition of OVA-induced airway eosinophilia, it did modulate OVA-specific immunoglobulin responses: oral administration of OVA alone suppressed OVA-specific IgG1 production, but only mice that received CpG-ODNs demonstrated enhanced levels of OVA-specific IgG2c. Finally, we examined whether oral administration of CpG-ODNs, alone or with OVA, could reverse established eosinophilic airway inflammation. Again, neither OVA nor CpG-ODNs alone modulated established eosinophilic airway inflammation, but a combination of the OVA and CpG-ODNs successfully desensitized the mice. This desensitization was associated with suppression of OVA-specific IgE and enhancement of OVA-specific IgG2c production. These findings provide the first indication that oral administration of CpG-ODNs is effective in preventing and reversing antigen-induced eosinophilic airway inflammation. CpG-ODNs may be useful as a component of oral immunotherapy to promote tolerance in established asthma.

Immunopathology

Fundamental and applied biological sciences. Psychology

Fundamental immunology

Biological and medical sciences

Medical sciences

Details

Title: Subtitle: Oral administration of CpG-ODNs suppresses antigen-induced asthma in mice
Creators: K KITAGAKI - Division of Pulmonary, Critical Care and Occupational Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, United States
T. R BUSINGA - Division of Pulmonary, Critical Care and Occupational Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, United States
J. N KLINE - Division of Pulmonary, Critical Care and Occupational Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, United States
Resource Type: Journal article
Publication Details: Clinical and experimental immunology, Vol.143(2), pp.249-259
DOI: 10.1111/j.1365-2249.2005.03003.x
PMID: 16412048
PMCID: PMC1809575
NLM abbreviation: Clin Exp Immunol
ISSN: 0009-9104
eISSN: 1365-2249
Publisher: Blackwell
Language: English
Date published: 2006
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Occupational and Environmental Health; Pathology; Internal Medicine
Record Identifier: 9984094633702771

Metrics

18 Record Views

35 Times Cited - Web of Science