Journal article
Orchestration of Stepwise Synaptic Growth by K+ and Ca2+ Channels in Drosophila
The Journal of neuroscience, Vol.30(47), pp.15821-15833
11/24/2010
DOI: 10.1523/JNEUROSCI.3448-10.2010
PMID: 21106821
Abstract
Synapse formation is tightly associated with neuronal excitability. We found striking synaptic overgrowth caused by
Drosophila
K
+
-channel mutations of the
seizure
and
slowpoke
genes, encoding Erg and Ca
2+
-activated large-conductance (BK) channels, respectively. These mutants display two distinct patterns of “satellite” budding from larval motor terminus synaptic boutons. Double-mutant analysis indicates that BK and Erg K
+
channels interact with separate sets of synaptic proteins to affect distinct growth steps. Post-synaptic L-type Ca
2+
channels, Dmca1D, and PSD-95-like scaffold protein, Discs large, are required for satellite budding induced by
slowpoke
and
seizure
mutations. Pre-synaptic
cacophony
Ca
2+
channels and the NCAM-like adhesion molecule, Fasciclin II, take part in a maturation step that is partially arrested by
seizure
mutations. Importantly,
slowpoke
and
seizure
satellites were both suppressed by
rutabaga
mutations that disrupt Ca
2+
/CaM-dependent adenylyl cyclase, demonstrating a convergence of K
+
channels of different functional categories in regulation of excitability-dependent Ca
2+
influx for triggering cAMP-mediated growth plasticity.
Details
- Title: Subtitle
- Orchestration of Stepwise Synaptic Growth by K+ and Ca2+ Channels in Drosophila
- Creators
- Jihye Lee - Interdisciplinary Program in Neuroscience andChun-Fang Wu - Interdisciplinary Program in Neuroscience and
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.30(47), pp.15821-15833
- Publisher
- Society for Neuroscience
- DOI
- 10.1523/JNEUROSCI.3448-10.2010
- PMID
- 21106821
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Language
- English
- Date published
- 11/24/2010
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070571202771
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