Journal article
Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds
Nature biomedical engineering, Vol.2(6), pp.443-452
06/2018
DOI: 10.1038/s41551-018-0231-0
PMCID: PMC6166886
PMID: 31011191
Abstract
Metastatic disease remains the primary cause of mortality in cancer patients. Yet the number of available in vitro models to study metastasis is limited by challenges in the recapitulation of the metastatic microenvironment in vitro, and by difficulties in maintaining colonized-tissue specificity in the expansion and maintenance of metastatic cells. Here, we show that decellularized scaffolds that retain tissue-specific extracellular-matrix components and bound signalling molecules enable, when seeded with colorectal cancer cells, the spontaneous formation of three-dimensional cell colonies that histologically, molecularly and phenotypically resemble in vivo metastases. Lung and liver metastases obtained by culturing colorectal cancer cells on, respectively, lung and liver decellularized scaffolds retained their tissue-specific tropism when injected in mice. We also found that the engineered metastases contained signet ring cells, which has not previously been observed ex vivo. A culture system with tissue-specific decellularized scaffolds represents a simple and powerful approach for the study of organ-specific cancer metastases.
Details
- Title: Subtitle
- Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds
- Creators
- Xi Tian - University of North Carolina at Chapel HillMichael E Werner - University of North Carolina at Chapel HillKyle C Roche - University of North Carolina at Chapel HillAriel D Hanson - University of North Carolina at Chapel HillHenry P Foote - University of North Carolina at Chapel HillStephanie K Yu - University of North Carolina at Chapel HillSamuel B Warner - University of North Carolina at Chapel HillJonathan A Copp - University of North Carolina at Chapel HillHaydee Lara - University of North Carolina at Chapel HillEliane L Wauthier - University of North Carolina at Chapel HillJoseph M Caster - University of North Carolina at Chapel HillLaura E Herring - University of North Carolina at Chapel HillLongzhen Zhang - Xuzhou Medical CollegeJoel E Tepper - University of North Carolina at Chapel HillDavid S Hsu - Duke Medical CenterTian Zhang - Duke Medical CenterLola M Reid - University of North Carolina at Chapel HillAndrew Z Wang - University of North Carolina at Chapel Hill
- Resource Type
- Journal article
- Publication Details
- Nature biomedical engineering, Vol.2(6), pp.443-452
- DOI
- 10.1038/s41551-018-0231-0
- PMID
- 31011191
- PMCID
- PMC6166886
- ISSN
- 2157-846X
- eISSN
- 2157-846X
- Grant note
- R21 CA182322 / NCI NIH HHS
- Language
- English
- Date published
- 06/2018
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984313073602771
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