Journal article
Osmotic regulation of estrogen receptor-beta expression in magnocellular vasopressin neurons requires lamina terminalis
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.286(3), pp.R465-473
03/2004
DOI: 10.1152/ajpregu.00478.2003
PMID: 14604844
Abstract
Estrogen receptor-beta (ER-beta) expression in rat magnocellular vasopressin (VP) neurons of the supraoptic and paraventricular nuclei (SON and PVN, respectively) becomes undetectable after 72 h of 2% NaCl consumption. To test the hypothesis that osmosensitive mechanisms that originate in the region of the organum vasculosum lamina terminalis (OVLT) control ER-beta expression in the SON and PVN, animals were water deprived after electrolytic lesions were performed on the area anterior to the ventral third ventricle (AV3V). Such lesions prevent osmotic stimulation of VP release. Four weeks after surgery, male rats [lesioned (n = 16) or sham (n = 14)] were water deprived for 48 h or allowed water ad libitum. Water deprivation eliminated ER-beta-immunoreactivity (-ir) in SON and magnocellular PVN of sham-lesioned animals. Fos-ir was evident in these neurons, and plasma osmolality (Posm) and hematocrit (Ht) were significantly elevated compared with the sham-hydrated rats (Posm, 304 +/- 1 vs. 318 +/- 2 mosmol/kgH2O; P < 0.001; Ht, 49.6 +/- 0.6 vs. 55.0 +/- 0.9%; P < 0.001). ER-beta expression was comparable in sham-hydrated, AV3V-hydrated, and 6 of 8 AV3V-dehydrated rats despite significant increases in Posm in both groups (AV3V hydrated, 312 +/- 2; AV3V dehydrated, 380 +/- 10 mosmol/kgH2O; P < 0.001). OVLT was not ablated in the AV3V-dehydrated rats in which ER-beta was depleted. Fos-ir was low or undetectable in SON in the AV3V-hydrated animals despite elevated Posm values. In AV3V-dehydrated rats, Fos-ir was significantly less than in sham-dehydrated animals but was significantly increased compared with the sham-hydrated group. This could reflect activation by nonosmotic parameters that do not inhibit ER-beta expression. These data support the hypothesis that inhibition of ER-beta expression in the SON by osmotic stimulation is mediated by osmoreceptive neurons in the lamina terminalis.
Details
- Title: Subtitle
- Osmotic regulation of estrogen receptor-beta expression in magnocellular vasopressin neurons requires lamina terminalis
- Creators
- Suwit J Somponpun - Department of Physiology and Biophysics, University of Colorado Health Science Center, Denver, Colorado 80262, USAAlan Kim JohnsonTerry BeltzCelia D Sladek
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.286(3), pp.R465-473
- DOI
- 10.1152/ajpregu.00478.2003
- PMID
- 14604844
- ISSN
- 0363-6119
- eISSN
- 1522-1490
- Grant note
- HL-14388 / NHLBI NIH HHS HL-57472 / NHLBI NIH HHS R01 NS-27975 / NINDS NIH HHS
- Language
- English
- Date published
- 03/2004
- Academic Unit
- Psychological and Brain Sciences; Neuroscience and Pharmacology; Health and Human Physiology
- Record Identifier
- 9984214757402771
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