Osteoblast integrin adhesion and signaling regulate mineralization

G B Schneider; R Zaharias; C Stanford

doi:10.1177/00220345010800061201

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Osteoblast integrin adhesion and signaling regulate mineralization

Journal article

Peer reviewed

Osteoblast integrin adhesion and signaling regulate mineralization

G B Schneider, R Zaharias and C Stanford

Journal of dental research, Vol.80(6), pp.1540-1544

06/2001

DOI: 10.1177/00220345010800061201

PMID: 11499509

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Abstract

Integrin adhesion and signaling events may contribute to the progressive differentiation of the osteoblast and to the initiation of a mineralized matrix. The purpose of our study was to begin to analyze the role of integrin receptors, in particular alpha2beta1, alpha5beta1, and alphaVbeta3, regarding mediation of the initiation of a mineralized matrix. Integrin-perturbation assays were conducted in microdot cultures of UMR-106-01 Bone Sialoprotein (BSP) osteoblast cells. For phenotypic analysis, we performed bright-field microscopy and Aliziran Red S staining to analyze effects on mineralization initiation. Mineralization was reduced significantly (P < 0.001) following the addition of alpha5- or beta1-integrin subunit antibody by approximately 20% and 45%, respectively--alphaVbeta3 integrin by nearly 65%, and alpha2beta1 integrin by nearly 95%. This effect was reversible following the removal of the antiintegrin antibody. These results suggest that integrin adhesion and signaling events may contribute to the ability of this cell line to mediate the initiation of the mineralization phenotype biologically.

Dose-Response Relationship, Immunologic

Signal Transduction

Integrin-Binding Sialoprotein

Rats

Integrins - metabolism

Tooth Calcification - genetics

Receptors, Cytoadhesin - metabolism

Animals

Sialoglycoproteins - physiology

Analysis of Variance

Cell Adhesion - physiology

Gene Expression Regulation, Developmental

Extracellular Matrix - physiology

Receptors, Collagen

Receptors, Fibronectin - metabolism

Cell Differentiation

Receptors, Vitronectin - metabolism

Tumor Cells, Cultured

Osteoblasts - metabolism

Osteosarcoma - metabolism

Protein Binding - physiology

Tooth Calcification - physiology

Details

Title: Subtitle: Osteoblast integrin adhesion and signaling regulate mineralization
Creators: G B Schneider - Dows Institute for Dental Research and the Department of Prosthodontics, University of Iowa College of Dentistry, Iowa City 52242, USA. galen-schneider@uiowa.edu
R Zaharias
C Stanford
Resource Type: Journal article
Publication Details: Journal of dental research, Vol.80(6), pp.1540-1544
DOI: 10.1177/00220345010800061201
PMID: 11499509
NLM abbreviation: J Dent Res
ISSN: 0022-0345
eISSN: 1544-0591
Publisher: United States
Grant note: P3010126-09 / PHS HHS P60DE13076 / NIDCR NIH HHS
Language: English
Date published: 06/2001
Academic Unit: Dentistry Administration; Prosthodontics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center
Record Identifier: 9984066081902771

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