Journal article
Osteoclasts are dispensable for hematopoietic progenitor mobilization by granulocyte colony-stimulating factor in mice
Experimental hematology, Vol.43(2), pp.110-114
02/01/2015
DOI: 10.1016/j.exphem.2014.10.012
PMCID: PMC4323843
PMID: 25461255
Abstract
The contribution of osteoclasts to hematopoietic stem/progenitor cell (HSPC) retention in the bone marrow is controversial. Studies of HSPC trafficking in osteoclast-deficient mice are limited by osteopetrosis. Here, we employed two non-osteopetrotic mouse models to assess the contribution of osteoclasts to basal and granulocyte colony-stimulating factor (G-CSF)-induced HSPC mobilization. We generated Rank(-/-) fetal liver chimeras using Csf3r(-/-) recipients to produce mice lacking G-CSF receptor expression in osteoclasts. Basal and G-CSF-induced HSPC mobilization was normal in these chimeras. We next acutely depleted osteoclasts in wild-type mice using the RANK ligand inhibitor osteoprotegerin. Marked suppression of osteoclasts was observed after a single injection of osteoprotegerin-Fc. Basal and G-CSF-induced HSPC mobilization in osteoprotegerin-Fc-treated mice was comparable to that in control mice. Together, these data indicate that osteoclasts are not required for the efficient retention of HSPCs in the bone marrow and are dispensable for HSPC mobilization by G-CSF. Copyright (C) 2015 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc.
Details
- Title: Subtitle
- Osteoclasts are dispensable for hematopoietic progenitor mobilization by granulocyte colony-stimulating factor in mice
- Creators
- Mahil Rao - Washington University in St. LouisTeerawit Supakomdej - Washington Univ, Sch Med, Dept Med, Div Oncol, St Louis, MO 63110 USAAmy P. Schmidt - Washington University in St. LouisDaniel C. Link - Washington University in St. Louis
- Resource Type
- Journal article
- Publication Details
- Experimental hematology, Vol.43(2), pp.110-114
- DOI
- 10.1016/j.exphem.2014.10.012
- PMID
- 25461255
- PMCID
- PMC4323843
- NLM abbreviation
- Exp Hematol
- ISSN
- 0301-472X
- eISSN
- 1873-2399
- Publisher
- Elsevier
- Number of pages
- 5
- Grant note
- RO1 HL60772 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01HL060772 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
- Language
- English
- Date published
- 02/01/2015
- Academic Unit
- Critical Care; Stead Family Department of Pediatrics
- Record Identifier
- 9984775023402771
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