Journal article
Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds
Biomaterials, Vol.31(6), pp.1133-1139
2010
DOI: 10.1016/j.biomaterials.2009.10.030
PMCID: PMC2813991
PMID: 19857889
Abstract
Amniotic fluid-derived stem cells (AFSCs) are becoming an important source of cells for regenerative medicine given their apparent advantages of accessibility, renewal capacity and multipotentiality. In the intermediate stage between the embryonic stem cells (ESCs) and adult stem cells, AFSCs may have a distinct mechanism to choose their fate. Unfortunately, until now, little is known about how bone morphogenetic proteins (BMPs) control the osteoblastic differentiation of AFSCs, especially on 3D scaffolds. Our research shows that human AFSCs (hAFSCs) can be induced for osteoblastic differentiation by rhBMP-7, and hAFSCs respond to rhBMP-7 more strongly than human mesenchymal stem cells (hMSCs). As synthetic ECM, scaffolds play a central role in tissue engineering. The hAFSCs, on the nanofibrous scaffolds (NF scaffolds) with morphology similar to that of natural collagen fibers, showed significantly enhanced alkaline phosphatase (ALP) activity, calcium content, von Kossa staining and the expression of osteogenic genes than those on the traditional scaffolds, i.e. solid walled scaffolds. The data on the bone formation
in vivo presented further evidence that biomimetic NF scaffolds provided hAFSCs a more favorable synthetic ECM, and thus, facilitated the osteogenic differentiation of hAFSCs. The relative strong responsiveness to rhBMP-7 makes hAFSCs promising in bone regeneration. The synthetic NF scaffolds, which mimic the morphology of natural collagen fibers, enhanced the osteoblastic differentiation of hAFSCs
in vitro and bone formation
in vivo.
Details
- Title: Subtitle
- Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds
- Creators
- Hongli Sun - University of MichiganKai Feng - University of MichiganJiang Hu - University of MichiganShay Soker - Wake Forest UniversityAnthony Atala - Forest InstitutePeter X. Ma - University of Michigan
- Resource Type
- Journal article
- Publication Details
- Biomaterials, Vol.31(6), pp.1133-1139
- DOI
- 10.1016/j.biomaterials.2009.10.030
- PMID
- 19857889
- PMCID
- PMC2813991
- NLM abbreviation
- Biomaterials
- ISSN
- 0142-9612
- eISSN
- 1878-5905
- Publisher
- Elsevier Ltd
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: DE015384, NIDCR DE017689
- Language
- English
- Date published
- 2010
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery
- Record Identifier
- 9984367722302771
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