Outdoor performance of Chlorococcum littorale at different locations

Iago Teles Dominguez Cabanelas; Petronella M. Slegers; Hanna Böpple; Dorinde M.M. Kleinegris; René H. Wijffels; Maria J. Barbosa

doi:10.1016/j.algal.2017.08.010

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Outdoor performance of Chlorococcum littorale at different locations

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Outdoor performance of Chlorococcum littorale at different locations

Iago Teles Dominguez Cabanelas, Petronella M. Slegers, Hanna Böpple, Dorinde M.M. Kleinegris, René H. Wijffels and Maria J. Barbosa

Algal research (Amsterdam), Vol.27, pp.55-64

11/01/2017

DOI: 10.1016/j.algal.2017.08.010

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Abstract

Our goal in the present study was to evaluate the potential for lipid production of two cell populations of the marine microalgae Chlorococcum littorale under different climate conditions. We selected, in a previous study and via fluorescence activated cell sorting (FACS), a new cell population of Chlorococcum littorale, namely S5. S5 showed a stable doubled triacylglycerol (TAG) productivity in comparison with the original population. A previously developed model was expanded to include day:night cycles and validated to predict biomass and outdoor TAG productivities at different locations. Four different locations were chosen to simulate the response of C. littorale to different day lengths and light intensities (the Netherlands, Norway, Brazil and Spain). Indoor experiments (simulated summer) were carried out with Original and S5, showing that S5 had a doubled TAG productivity under N-starvation. Finally, simulations of biomass and TAG productivities of Original and S5 at different locations were performed. At locations with lower light intensities, Norway and the Netherlands, biomass productivities were higher than at locations with higher light intensities, Brazil/Spain. Such results might be associated with light-saturation effects. TAG productivities, however, showed no effect of local light intensity. Locations at higher latitudes, Norway/Netherlands, cannot sustain phototrophic year-round production, hence, the yearly average TAG productivities were doubled in Brazil/Spain (from 1.4–1.6 to 3.0–3.2gm−2d−1). Likewise, C. littorale S5 was simulated with doubled TAG productivities when compared with Original, at all locations (2.5–2.7 (low light) to 4.7–5.2gm−2d−1 (high light)). The present results confirm the industrial potential of Chlorococcum littorale, both Original and S5, as a source of TAG. Furthermore, our results can be used for comparison and to estimate future production scenarios. •Carbon partitioning (starch/triacylglycerols (TAG)) of N-starved C. littorale is described.•Two strains of C. littorale were studied: the original strain and a sorted strain (S5).•Successful simulations confirmed doubled TAG yield of S5 compared to Original.•High irradiance locations show lower biomass yields on light compared to low irradiance.•TAG yields were not affected by location-dependent irradiance intensity

Chlorococcum Littorale

Microalgae

Modelling carbon partitioning

Outdoor productivities

Strain improvement

Tag

Year round productivities

Details

Title: Subtitle: Outdoor performance of Chlorococcum littorale at different locations
Creators: Iago Teles Dominguez Cabanelas - Wageningen University & Research
Petronella M. Slegers - Wageningen University & Research
Hanna Böpple - Uni Research (Norway)
Dorinde M.M. Kleinegris - Uni Research (Norway)
René H. Wijffels - Wageningen University & Research
Maria J. Barbosa - Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 16, 6700 AA Wageningen, Netherlands11www.AlgaePARC.com
Resource Type: Journal article
Publication Details: Algal research (Amsterdam), Vol.27, pp.55-64
DOI: 10.1016/j.algal.2017.08.010
ISSN: 2211-9264
eISSN: 2211-9264
Publisher: Elsevier B.V
Grant note: name: Ministry of Economic Affairs, Agriculture and Innovation, award: TRCDKI/2010/0956; name: Province of Gelderland; DOI: 10.13039/100004349, name: BASF; name: BioOils; name: Drie Wilgen Development; DOI: 10.13039/100010408, name: DSM; DOI: 10.13039/100004371, name: Exxon Mobil; name: GEA Westfalia Separator; name: Heliae; name: Neste; name: Nijhuis; name: Paques; name: Cellulac; name: Proviron; name: Roquette; DOI: 10.13039/501100007164, name: SABIC; name: Simris Alg; name: Staatsolie Suriname; name: Synthetic Genomics; DOI: 10.13039/501100007185, name: TOTAL; DOI: 10.13039/100007190, name: Unilever; DOI: 10.13039/501100003141, name: National Council of Science and Technology, award: 236614/2012-6
Language: English
Date published: 11/01/2017
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984398029402771

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