Journal article
Overexpression of Bcl-2 or Bcl-xL prevents spiral ganglion neuron death and inhibits neurite growth
Developmental neurobiology (Hoboken, N.J.), Vol.67(3), pp.316-325
02/15/2007
DOI: 10.1002/dneu.20346
PMID: 17443790
Abstract
Spiral ganglion neurons (SGNs) provide afferent innervation to the cochlea and rely on contact with hair cells (HCs) for their survival. Following deafferentation due to hair cell loss, SGNs gradually die. In a rat culture model, we explored the ability of prosurvival members of the Bcl-2 family of proteins to support the survival and neurite outgrowth of SGNs. We found that overexpression of either Bcl-2 or Bcl-xL significantly increases SGN survival in the absence of neurotrophic factors, establishing that the Bcl-2 pathway is sufficient for SGN cell survival and that SGN deprived of trophic support die by an apoptotic mechanism. However, in contrast to observations in central neurons and PC12 cells where Bcl-2 appears to promote neurite growth, both Bcl-2 and Bcl-xL overexpression dramatically inhibit neurite outgrowth in SGNs. This inhibition of neurite growth by Bcl-2 occurs in nearly all SGNs even in the presence of multiple neurotrophic factors implying that Bcl-2 directly inhibits neurite growth rather than simply rescuing a subpopulation of neurons incapable of extending neurites without additional stimuli. Thus, although overexpression of prosurvival members of the Bcl-2 family prevents SGN loss following trophic factor deprivation, the inhibition of neurite growth by these molecules may limit their efficacy for support of auditory nerve maintenance or regeneration following hair cell loss.
Details
- Title: Subtitle
- Overexpression of Bcl-2 or Bcl-xL prevents spiral ganglion neuron death and inhibits neurite growth
- Creators
- Marlan R Hansen - Department of Otolaryngology, Head, and Neck Surgery, University of Iowa, Iowa City, Iowa 52242, USA. marlan-hansen@uiowa.eduPamela C RoehmNingyong XuSteven H Green
- Resource Type
- Journal article
- Publication Details
- Developmental neurobiology (Hoboken, N.J.), Vol.67(3), pp.316-325
- Publisher
- United States
- DOI
- 10.1002/dneu.20346
- PMID
- 17443790
- ISSN
- 1932-8451
- eISSN
- 1932-846X
- Grant note
- KO8 DC006211 / NIDCD NIH HHS R01 DC02961 / NIDCD NIH HHS R01 DC002961 / NIDCD NIH HHS
- Language
- English
- Date published
- 02/15/2007
- Academic Unit
- Molecular Physiology and Biophysics; Biology; Neurosurgery; Otolaryngology
- Record Identifier
- 9984006323902771
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