Journal article
Overexpression of manganese or copper–zinc superoxide dismutase inhibits breast cancer growth
Free radical biology & medicine, Vol.41(2), pp.226-237
2006
DOI: 10.1016/j.freeradbiomed.2006.03.015
PMID: 16814103
Abstract
We have studied the effects of overexpression of superoxide dismutase (SOD), a tumor suppressor protein that dismutes superoxide radical to H
2O
2, on breast cancer cell growth in vitro and xenograft growth in vivo. No previous work has directly compared the growth-suppressive effects of manganese SOD (MnSOD) and copper–zinc SOD (CuZnSOD). We hypothesized that either adenoviral MnSOD (Ad
MnSOD) or adenoviral CuZnSOD (Ad
CuZnSOD) gene therapy would suppress the growth of human breast cancer cells. After determining the antioxidant profiles of three human breast cell lines, MCF 10A, MDA-MB231, and MCF-7, we measured the effects of MnSOD or CuZnSOD overexpression on cell growth and survival in vitro and in vivo. Results demonstrated that infection with Ad
MnSOD or Ad
CuZnSOD increased the activity of the respective enzyme in all three cell lines. In vitro, overexpression of MnSOD or CuZnSOD decreased not only cell growth but also clonogenic survival in a dose- and transgene-dependent manner. In vivo, treatment of tumors with Ad
MnSOD or Ad
CuZnSOD decreased xenograft growth compared to controls. The first direct comparison of MnSOD to CuZnSOD overexpression indicated that CuZnSOD and MnSOD were similarly effective at suppressing cancer cell growth.
Details
- Title: Subtitle
- Overexpression of manganese or copper–zinc superoxide dismutase inhibits breast cancer growth
- Creators
- Christine J. Weydert - Roy J. and Lucille A. Carver College of MedicineTrent A. Waugh - Roy J. and Lucille A. Carver College of MedicineJustine M. Ritchie - University of IowaKanchan S. Iyer - University of IowaJenna L. Smith - University of IowaLing Li - Roy J. and Lucille A. Carver College of MedicineDouglas R. Spitz - University of IowaLarry W. Oberley - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.41(2), pp.226-237
- DOI
- 10.1016/j.freeradbiomed.2006.03.015
- PMID
- 16814103
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2006
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984312987702771
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