Oxadiargyl induced conformational transition of cystatin isolated from yellow mustard seeds: Biophysical and biochemical approach

Azaj Ahmed; Anas Shamsi; Bilqees Bano

doi:10.1016/j.ijbiomac.2017.02.055

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Oxadiargyl induced conformational transition of cystatin isolated from yellow mustard seeds: Biophysical and biochemical approach

Journal article

Peer reviewed

Oxadiargyl induced conformational transition of cystatin isolated from yellow mustard seeds: Biophysical and biochemical approach

Azaj Ahmed, Anas Shamsi and Bilqees Bano

International journal of biological macromolecules, Vol.98, pp.802-809

05/2017

DOI: 10.1016/j.ijbiomac.2017.02.055

PMID: 28214581

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Abstract

Phytocystatins are thiol proteinase inhibitors crucial due to their inhibitory activity in plants. These play important roles in improving crop yield, protection against insects and pathogens and modulation of apoptosis. In this chemical era, various pesticides are being used globally to increase the crop biomass. These pesticides accumulate in plant body and produce harmful effects on plants itself by interacting with essential proteins. In this present study, we have monitored the interaction of a herbicide; oxadiargyl, with phytocystatin isolated from yellow mustard seeds (YMP) by employing spectroscopic techniques viz. UV, fluorescence, FTIR and CD spectroscopy and Isothermal titration calorimetry (ITC). UV and fluorescence spectroscopy shows YMP transformation from native to non-native form apparent by decreased absorbance and decreased fluorescence. FTIR and CD spectroscopy further confirmed secondary structural disruption of YMP. Anti-papain activity assay was also carried out; a reduction in activity was observed in presence of oxadiargyl. Thermodynamic parameters obtained from ITC and stern-volmer plot shows affinity of oxadiargyl towards phytocystatin. Oxadiargyl was also found to induce ROS generation in YMP as evident by DNPH assay. Thus oxadiargyl binds to phytocystatin causing structural alterations reducing its physiological benefits and altering its functionality and ultimately leading to reduced crop yield.

Biophysical Phenomena

Cystatins - chemistry

Molecular Conformation - drug effects

Mustard Plant - chemistry

Oxadiazoles - chemistry

Oxadiazoles - pharmacology

Seeds - chemistry

Thermodynamics

Details

Title: Subtitle: Oxadiargyl induced conformational transition of cystatin isolated from yellow mustard seeds: Biophysical and biochemical approach
Creators: Azaj Ahmed - Aligarh Muslim University
Anas Shamsi - Aligarh Muslim University
Bilqees Bano - Aligarh Muslim University
Resource Type: Journal article
Publication Details: International journal of biological macromolecules, Vol.98, pp.802-809
DOI: 10.1016/j.ijbiomac.2017.02.055
PMID: 28214581
ISSN: 0141-8130
eISSN: 1879-0003
Language: English
Date published: 05/2017
Academic Unit: Internal Medicine
Record Identifier: 9985178665602771

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