Journal article
Oxidative stress augments chemoreflex sensitivity in rats exposed to chronic intermittent hypoxia
Respiratory physiology & neurobiology, Vol.234, pp.47-59
12/2016
DOI: 10.1016/j.resp.2016.09.001
PMCID: PMC5096995
PMID: 27595979
Abstract
•Chronic intermittent hypoxia (CIH) augmented the hypoxic ventilatory response.•This increase was prevented by pharmacological inhibitors of superoxide production.•Superoxide inhibition prevented the CIH-induced drop in metabolic rate.•CIH increased carotid body nitrotyrosine production.
Chronic exposure to intermittent hypoxia (CIH) elicits plasticity of the carotid sinus and phrenic nerves via reactive oxygen species (ROS). To determine whether CIH-induced alterations in ventilation, metabolism, and heart rate are also dependent on ROS, we measured responses to acute hypoxia in conscious rats after 14 and 21 d of either CIH or normoxia (NORM), with or without concomitant administration of allopurinol (xanthine oxidase inhibitor), combined allopurinol plus losartan (angiotensin II type 1 receptor antagonist), or apocynin (NADPH oxidase inhibitor). Carotid body nitrotyrosine production was measured by immunohistochemistry. CIH produced an increase in the ventilatory response to acute hypoxia that was virtually eliminated by all three pharmacologic interventions. CIH caused a robust increase in carotid body nitrotyrosine production that was greatly attenuated by allopurinol plus losartan and by apocynin but unaffected by allopurinol. CIH caused a decrease in metabolic rate and a reduction in hypoxic bradycardia. Both of these effects were prevented by allopurinol, allopurinol plus losartan, and apocynin.
Details
- Title: Subtitle
- Oxidative stress augments chemoreflex sensitivity in rats exposed to chronic intermittent hypoxia
- Creators
- Barbara J Morgan - John Rankin Laboratory of Pulmonary Medicine, School of Medicine and Public Health: University of Wisconsin, Madison, WI 53706, USAMelissa L Bates - Department of Health and Human Physiology, College of Liberal Arts and Sciences; University of Iowa, Iowa City, IA 52242, USARodrigo Del Rio - Laboratory of Cardiorespiratory Control, Universidad Autónoma de Chile, Santiago, ChileZunyi Wang - Department of Surgical Sciences, School of Veterinary Medicine; University of Wisconsin, Madison, WI 53706, USAJohn M Dopp - Pharmacy Practice Division, School of Pharmacy; University of Wisconsin, Madison, WI 53705, USA
- Resource Type
- Journal article
- Publication Details
- Respiratory physiology & neurobiology, Vol.234, pp.47-59
- DOI
- 10.1016/j.resp.2016.09.001
- PMID
- 27595979
- PMCID
- PMC5096995
- NLM abbreviation
- Respir Physiol Neurobiol
- ISSN
- 1569-9048
- eISSN
- 1878-1519
- Publisher
- Elsevier BV
- Language
- English
- Date published
- 12/2016
- Academic Unit
- Stead Family Department of Pediatrics; Health, Sport, and Human Physiology ; Internal Medicine
- Record Identifier
- 9984002356702771
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