Journal article
Oxygen free radicals mediate the induction of manganese superoxide dismutase gene expression by TNF-α
Free radical biology & medicine, Vol.28(8), pp.1197-1205
2000
DOI: 10.1016/S0891-5849(00)00237-9
PMID: 10889449
Abstract
In this study, the hypothesis that oxygen free radicals act as intracellular messengers is examined. Treatment of human oral carcinoma SCC-25 cells with 200 ng/ml human TNF-α for 6 h greatly increased manganese superoxide dismutase (MnSOD) gene expression as detected by western blotting, RT-PCR, and nuclear run-on experiments. In the presence of the oxygen free radical spin trapping reagent, 5,5-dimethyl pyrroline-
N-oxide (DMPO), the induction of MnSOD gene expression by TNF-α was significantly reduced. Electron paramagnetic resonance experiments showed that the production of oxygen free radicals was enhanced in TNF-α treated cells. Taken together, these observations suggest that the induction of MnSOD expression by TNF-α is at least partially mediated by intracellular formation of oxygen free radicals, and that superoxide is most likely the initiating species involved in the mediation of MnSOD gene expression by TNF-α.
Details
- Title: Subtitle
- Oxygen free radicals mediate the induction of manganese superoxide dismutase gene expression by TNF-α
- Creators
- Rugao Liu - Radiation Research Laboratory, The University of Iowa, Iowa City, IA, USAGarry R Buettner - Radiation Research Laboratory, The University of Iowa, Iowa City, IA, USALarry W Oberley - Radiation Research Laboratory, The University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.28(8), pp.1197-1205
- DOI
- 10.1016/S0891-5849(00)00237-9
- PMID
- 10889449
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2000
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047708202771
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