Journal article
Oxygen toxicity in control and H 2O 2-resistant Chinese hamster fibroblast cell lines
Archives of biochemistry and biophysics, Vol.279(2), pp.249-260
1990
DOI: 10.1016/0003-9861(90)90489-L
Abstract
Following exposure to 95% oxygen, clonogenic cell survival was assayed and qualitative morphologic changes were observed in a Chinese hamster fibroblast cell line (HA-1). The time in 95% O
2 necessary to clonogenically inactivate 90% of the cells was inversely related to the cell density of the cultures at the beginning of hyperoxic exposure (from 1 to 6 × 10
4 cells/cm
2). The O
2-induced loss in clonogenicity and evidence of morphologic injury were shown to be significantly delayed (17–22 h) in an H
2O
2-resistant variant of the parental HA-1 cell line. After the delay in onset of clonogenic cell killing or morphologic injury, the process of injury proceeded in a similar fashion in both cell lines. The H
2O
2-resistant cell line demonstrated significantly greater catalase activity (20-fold), CuZn superoxide dismutase activity (2-fold), and Se-dependent glutathione peroxidase activity (1.5-fold). The greater activities of CuZn superoxide dismutase and catalase were accompanied by similarly greater quantities of immunoreactive protein as determined by immunoblotting. These data demonstrate that the cells adapted and/or selected for growth in a highly peroxidative environment also became refractory to O
2-induced toxicity, which may be related to increased expression of antioxidant enzymes. However, the magnitude of this cross-resistance to O
2 toxicity was less than the magnitude of the cellular resistance to the toxicity of exogenous H
2O
2, suggesting that in this system the toxicity of 95% oxygen is not identical to H
2O
2-mediated cytotoxicity.
Details
- Title: Subtitle
- Oxygen toxicity in control and H 2O 2-resistant Chinese hamster fibroblast cell lines
- Creators
- Douglas R. Spitz - University of Virginia HospitalJames H. Elwell - University of IowaYi Sun - University of IowaLarry W. Oberley - University of IowaTerry D. Oberley - University of Wisconsin–MadisonShannon J. Sullivan - University of Virginia HospitalRobert J. Roberts - University of Virginia Hospital
- Resource Type
- Journal article
- Publication Details
- Archives of biochemistry and biophysics, Vol.279(2), pp.249-260
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0003-9861(90)90489-L
- ISSN
- 0003-9861
- eISSN
- 1096-0384
- Language
- English
- Date published
- 1990
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Stead Family Department of Pediatrics
- Record Identifier
- 9984312986002771
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