Journal article
P-TEFb kinase recruitment and function at heat shock loci
Genes & development, Vol.14(7), pp.792-803
04/01/2000
DOI: 10.1101/gad.14.7.792
PMID: 10766736
Abstract
P-TEFb, a heterodimer of the kinase Cdk9 and cyclin T, was isolated as a factor that stimulates formation of productive transcription elongation complexes in vitro. Here, we show that P-TEFb is located at >200 distinct sites on
Drosophila
polytene chromosomes. Upon heat shock, P-TEFb, like the regulatory factor HSF, is rapidly recruited to heat shock loci, and this recruitment is blocked in an HSF mutant. Yet, HSF binding to DNA is not sufficient to recruit P-TEFb in vivo, and HSF and P-TEFb immunostainings within a heat shock locus are not coincident. Insight to the function of P-TEFb is offered by experiments showing that the direct recruitment of a Gal4-binding domain P-TEFb hybrid to an
hsp70
promoter in
Drosophila
cells is sufficient to activate transcription in the absence of heat shock. Analyses of point mutants show this P-TEFb stimulation is dependent on Cdk9 kinase activity and on Cdk9's interaction with cyclin T. These results, coupled with the frequent colocalization of P-TEFb and the hypophosphorylated form of RNA polymerase II (Pol II) found at promoter-pause sites, support a model in which P-TEFb acts to stimulate promoter-paused Pol II to enter into productive elongation.
Details
- Title: Subtitle
- P-TEFb kinase recruitment and function at heat shock loci
- Creators
- John T Lis - Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14853 USAPaul Mason - Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14853 USAJ Peng - Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14853 USADavid H Price - Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14853 USAJanis Werner - Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14853 USA
- Resource Type
- Journal article
- Publication Details
- Genes & development, Vol.14(7), pp.792-803
- Publisher
- Cold Spring Harbor Laboratory Press
- DOI
- 10.1101/gad.14.7.792
- PMID
- 10766736
- ISSN
- 0890-9369
- eISSN
- 1549-5477
- Language
- English
- Date published
- 04/01/2000
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984024504502771
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