Journal article
P122. Sox2 dosage defines development and survival of sensory neurons in the inner ear
Differentiation (London), Vol.80, pp.S58-S58
2010
DOI: 10.1016/j.diff.2010.09.128
Abstract
Inner ear function requires coordinated development of hair cells, supporting cells, and sensory neurons; cell types that share some common progenitors.
Sox2 is required for development of the first two, but its role in otic neurogenesis
in vivo is unknown. Here we show that
Sox2 is expressed in delaminating otic neuroblasts with
Neurogenin1 (
Neurog1), a gene required for their specification, and in differentiating sensory neurons. We therefore studied the genesis and maintenance of these neurons in mice carrying null (
Sox2β
geo
) and regulatory (
Sox2
Lcc
and
Sox2
Ysb
) mutations. In
Sox2
Lcc/Lcc
, where sensory epithelia do not form, we found a progressive reduction in
Sox2 expression, in the number of
Sox2 positive cells and relatively fewer delaminating neuroblasts in the developing otocyst during the critical stages of neurogenesis.
Neurog1 is expressed in
Sox2
Lcc/Lcc
and
Sox2 is expressed in
Neurog1 null embryos, suggesting that these two factors do not depend on each other. However, they may work together since relatively fewer cells expressing
Neurogenic differentiation 1 (
Neurod1), a known target of
Neurog1, were present in the
Sox2
Lcc/Lcc
otic epithelium. Although some sensory neurons differentiate in
Sox2
Lcc/Lcc
and extend their processes to areas of the developing ear where sensory epithelia would normally develop, these neurons soon disappear, probably because brain-derived neurotrophic factor (
Bdnf) is not expressed in the
Sox2
Lcc/Lcc
otic epithelium. These studies, together with those on heterozygous null and the hypomorphic
Sox2
Ybs/Ysb
mutants, allow us to conclude that
Sox2 levels control both specification of the full complement of otic neurons and maintenance of innervation.
Details
- Title: Subtitle
- P122. Sox2 dosage defines development and survival of sensory neurons in the inner ear
- Creators
- Kathryn S.E Cheah - Department of Biochemistry & Centre for Reproduction, Development & Growth, The University of Hong Kong, Li Ka Shing Faculty of Medicine Building, 21 Sassoon Road, Hong Kong, ChinaKeith K.H Leung - Department of Biochemistry & Centre for Reproduction, Development & Growth, The University of Hong Kong, Li Ka Shing Faculty of Medicine Building, 21 Sassoon Road, Hong Kong, ChinaAnna Pelling - Department of Biochemistry & Centre for Reproduction, Development & Growth, The University of Hong Kong, Li Ka Shing Faculty of Medicine Building, 21 Sassoon Road, Hong Kong, ChinaIsrat Jahan - Department of Biology, CLAS, University of Iowa, Iowa City, IA 52242-1324, USARobin Lovell-Badge - Division of Stem Cell Biology and Developmental Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UKBernd Fritzsch - Department of Biology, CLAS, University of Iowa, Iowa City, IA 52242-1324, USA
- Resource Type
- Journal article
- Publication Details
- Differentiation (London), Vol.80, pp.S58-S58
- DOI
- 10.1016/j.diff.2010.09.128
- ISSN
- 0301-4681
- eISSN
- 1432-0436
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 2010
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984071779202771
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