P1,P4-Di(adenosine-5')tetraphosphate inhibits phosphorylation of immunoglobulin G by Rous sarcoma virus pp60src

P F Maness; M E Perry; B T Levy

doi:10.1016/S0021-9258(18)32580-8

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P1,P4-Di(adenosine-5')tetraphosphate inhibits phosphorylation of immunoglobulin G by Rous sarcoma virus pp60src

Journal article

Open access

Peer reviewed

P1,P4-Di(adenosine-5')tetraphosphate inhibits phosphorylation of immunoglobulin G by Rous sarcoma virus pp60src

P F Maness, M E Perry and B T Levy

The Journal of biological chemistry, Vol.258(7), pp.4055-4058

04/10/1983

DOI: 10.1016/S0021-9258(18)32580-8

PMID: 6300072

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Abstract

Di(adenosine-5')oligophosphate nucleotides of general structure ApnA (n = 2-6) inhibited phosphorylation of immunoglobulin G from tumor-bearing rabbits (TBR IgG) by pp60src protein kinase purified from Rous sarcoma virus-transformed rat tumor cells. Ap4A, a nucleotide associated with eukaryotic cell proliferation, was one of the most effective inhibitors in the series, causing 50% inhibition of TBR IgG phosphorylation at 15 microM. Ap4A inhibited pp60src-dependent phosphorylation of TBR IgG in solution and immunoprecipitates, as well as the phosphorylation of tubulin, microtubule-associated proteins, and vinculin. Under similar assay conditions, Ap4A did not inhibit phosphorylation of histone H2b by cAMP- or cGMP-dependent protein kinases. Ap4A appears to interact noncovalently with the enzyme, because removal of pp60src by immunoprecipitation from solutions containing Ap4A restored activity to uninhibited levels. A 100-fold increase in ATP (4-400 nM) caused a 13-fold increase in the 50% inhibitory concentration of Ap4A (2.5-33 microM), consistent with the interpretation that Ap4A competes for an ATP-binding site on the pp60src molecule. The simplest explanation of these results is that Ap4A binds to the phosphodonor site for ATP.

Phosphorylation

Protein Kinases - metabolism

Cell Line

Cyclic GMP - pharmacology

Rabbits

Adenine Nucleotides - pharmacology

Oncogene Protein pp60(v-src)

Rats

Viral Proteins - metabolism

Cyclic AMP - pharmacology

Animals

Cell Transformation, Neoplastic

Neoplasms, Experimental - immunology

Viral Proteins - isolation & purification

Kinetics

Dinucleoside Phosphates

Immunoglobulin G - metabolism

Details

Title: Subtitle: P1,P4-Di(adenosine-5')tetraphosphate inhibits phosphorylation of immunoglobulin G by Rous sarcoma virus pp60src
Creators: P F Maness
M E Perry
B T Levy
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.258(7), pp.4055-4058
DOI: 10.1016/S0021-9258(18)32580-8
PMID: 6300072
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: NCI 2-P01-CA19014 / NCI NIH HHS
Language: English
Date published: 04/10/1983
Academic Unit: Epidemiology; Family and Community Medicine
Record Identifier: 9984025254902771

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