Journal article
P2X3 receptors induced inflammatory nociception modulated by TRPA1, 5-HT3 and 5-HT1A receptors
Pharmacology, biochemistry and behavior, Vol.112, pp.49-55
11/01/2013
DOI: 10.1016/j.pbb.2013.09.017
PMID: 24120766
Abstract
It has been described that endogenous ATP via activation of P2X3 and P2X2/3 receptors contributes to inflammatory nociception in different models, including the formalin injected in subcutaneous tissue of the rat's hind paw. In this study, we have evaluated whether TRPA1, 5-HT3 and 5-HT1A receptors, whose activation is essential to formalin-induced inflammatory nociception, are involved in the nociception induced by activation of P2X3 receptors on subcutaneous tissue of the rat's hind paw. We have also evaluated whether the activation of P2X3 receptors increases the susceptibility of primary afferent neurons to formalin action modulated by activation of TRPA1, 5-HT3 or 5-HT1A receptors. Nociceptive response intensity was measured by observing the rat's behavior and considering the number of times the animal reflexively raised its hind paw (flinches) in 60min. Local subcutaneous administration of the selective TRPA1, 5-HT3 or 5-HT1A receptor antagonists HC 030031, tropisetron and WAY 100,135, respectively, prevented the nociceptive responses induced by the administration in the same site of the non-selective P2X3 receptor agonist αβmeATP. Administration of the selective P2X3 and P2X2/3 receptor antagonist A-317491 or pretreatment with oligonucleotides antisense against P2X3 receptor prevented the formalin-induced behavioral nociceptive responses during the first and second phases. Also, the co-administration of a subthreshold dose of αβmeATP with a subthreshold dose of formalin induced nociceptive behavior, which was prevented by local administration of tropisetron, HC 030031 or WAY 100, 135. These findings have demonstrated that the activation of P2X3 receptors induces inflammatory nociception modulated by TRPA1, 5-HT3 and 5-HT1A receptors. Also, they suggest that inflammatory nociception is modulated by the release of endogenous ATP and P2X3 receptor activation, which in turn, increases primary afferent nociceptor susceptibility to the action of inflammatory mediators via interaction with TRPA1, 5-HT3 and 5-HT1A receptors in the peripheral tissue.
•Activation of peripheral P2X3 receptors induced inflammatory nociception.•This response was modulated by local TRPA1, 5-HT3 and 5-HT1A receptors.•P2X3 increases susceptibility of nociceptors to formalin-induced nociception.•This response is also modulated by TRPA1, 5-HT3 and 5-HT1A receptors.
Details
- Title: Subtitle
- P2X3 receptors induced inflammatory nociception modulated by TRPA1, 5-HT3 and 5-HT1A receptors
- Creators
- Suzy Krimon - Universidade Estadual de Campinas (UNICAMP)Dionéia Araldi - Universidade Estadual de Campinas (UNICAMP)Filipe César do Prado - Universidade Estadual de Campinas (UNICAMP)Cláudia Herrera Tambeli - Universidade Estadual de Campinas (UNICAMP)Maria Cláudia G. Oliveira-Fusaro - Universidade Estadual de Campinas (UNICAMP)Carlos Amílcar Parada - Universidade Estadual de Campinas (UNICAMP)
- Resource Type
- Journal article
- Publication Details
- Pharmacology, biochemistry and behavior, Vol.112, pp.49-55
- DOI
- 10.1016/j.pbb.2013.09.017
- PMID
- 24120766
- NLM abbreviation
- Pharmacol Biochem Behav
- ISSN
- 0091-3057
- eISSN
- 1873-5177
- Publisher
- Elsevier Inc
- Number of pages
- 7
- Language
- English
- Date published
- 11/01/2013
- Academic Unit
- Neuroscience and Pharmacology
- Record Identifier
- 9985178666902771
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