PAMAM dendrimers as nano carriers to investigate inflammatory responses induced by pulmonary exposure of PCB metabolites in Sprague-Dawley rats

Orarat Wangpradit; Andrea Adamcakova-Dodd; Katharina Heitz; Larry Robertson; Peter S Thorne; Gregor Luthe

doi:10.1007/s11356-015-5022-5

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PAMAM dendrimers as nano carriers to investigate inflammatory responses induced by pulmonary exposure of PCB metabolites in Sprague-Dawley rats

Journal article

Peer reviewed

PAMAM dendrimers as nano carriers to investigate inflammatory responses induced by pulmonary exposure of PCB metabolites in Sprague-Dawley rats

Orarat Wangpradit, Andrea Adamcakova-Dodd, Katharina Heitz, Larry Robertson, Peter S Thorne and Gregor Luthe

Environmental science and pollution research international, Vol.23(3), pp.2128-2137

02/2016

DOI: 10.1007/s11356-015-5022-5

PMCID: PMC4803647

PMID: 26400242

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Abstract

Polychlorinated biphenyls (PCBs) persist and accumulate in the ecosystem depending upon the degree of chlorination of the biphenyl rings. Airborne PCBs are especially susceptible to oxidative metabolism, yielding mono- and di-hydroxy metabolites. We have previously demonstrated that 4-chlorobiphenyl hydroquinones (4-CB-HQs) acted as cosubstrates for arachidonic acid metabolism by prostaglandin H synthase (PGHS) and resulted in an increase of prostaglandin production in vitro. In the present study, we tested the capability of 4-CB-HQ to act as a co-substrate for PGHS catalysis in vivo. BQ and 4-CB-2',5'-HQ were administered intratracheally to male Sprague-Dawley rats (2.5 μmol/kg body weight) using nanosized polyamidoamine (PAMAM) dendrimers as carriers. We found that 24 h post application, PGE2 metabolites in kidney of rats treated with 4-CB-2',5'-HQ were significantly increased compared to the controls. The increase of PGE2 metabolites was correlated with increased alveolar macrophages in lung lavage fluid. The elevation of PGE2 synthesis is of great interest since it plays a crucial role in balancing homeostasis and inflammation where a chronic disturbance may increase risk of cancer. PAMAM dentrimers proved to be an effective transport medium and did not stimulate an inflammatory response themselves.

Kidney - drug effects

Kidney - immunology

Rats

Male

Polychlorinated Biphenyls - metabolism

Rats, Sprague-Dawley

Prostaglandin-Endoperoxide Synthases - immunology

Macrophages, Alveolar - enzymology

Animals

Polychlorinated Biphenyls - chemistry

Polychlorinated Biphenyls - toxicity

Macrophages, Alveolar - drug effects

Lung - drug effects

Dendrimers - chemistry

Prostaglandins - immunology

Lung - metabolism

Lung - immunology

Macrophages, Alveolar - immunology

Details

Title: Subtitle: PAMAM dendrimers as nano carriers to investigate inflammatory responses induced by pulmonary exposure of PCB metabolites in Sprague-Dawley rats
Creators: Orarat Wangpradit - Sirindhorn College of Public Health, Bansuan, Muang, Chonburi, 20000, Thailand
Andrea Adamcakova-Dodd - Pulmonary Toxicology Facility, Environmental Health Science Research Center, The University of Iowa, UI Research Park, Iowa City, IA, 52242, USA
Katharina Heitz - Department of Occupational and Environmental Health, The University of Iowa, UI Research Park, Iowa City, IA, 52242, USA
Larry Robertson - Department of Occupational and Environmental Health, The University of Iowa, UI Research Park, Iowa City, IA, 52242, USA
Peter S Thorne - Pulmonary Toxicology Facility, Environmental Health Science Research Center, The University of Iowa, UI Research Park, Iowa City, IA, 52242, USA
Gregor Luthe - Luthe-Pharma, Fabrikstrasse 3, 48599, Gronau, Germany. g.luthe@luthe-pharma.com
Resource Type: Journal article
Publication Details: Environmental science and pollution research international, Vol.23(3), pp.2128-2137
DOI: 10.1007/s11356-015-5022-5
PMID: 26400242
PMCID: PMC4803647
NLM abbreviation: Environ Sci Pollut Res Int
ISSN: 0944-1344
eISSN: 1614-7499
Publisher: Germany
Grant note: P30 ES005605 / NIEHS NIH HHS P30 ES05605 / NIEHS NIH HHS P42 ES013661 / NIEHS NIH HHS P30 DK054759 / NIDDK NIH HHS
Language: English
Date published: 02/2016
Academic Unit: Civil and Environmental Engineering; Occupational and Environmental Health; Stead Family Department of Pediatrics; Iowa Superfund Research Program
Record Identifier: 9983997453302771

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