Journal article
PD-L1 expression in tumor cells and the immunologic milieu of bladder carcinomas: a pathologic review of 165 cases
Human pathology, Vol.81, pp.184-191
11/2018
DOI: 10.1016/j.humpath.2018.06.028
PMID: 29969606
Abstract
Programmed death ligand 1 (PD-L1) is a transmembrane protein that plays a major role in immune suppression. Its interaction with the receptor PD-1 results in downregulation of antitumoral immunity. Humanized monoclonal antibodies that interrupt the PD-L1/PD-1 interaction have shown therapeutic efficacy in patients with advanced urothelial cancer. However, immunohistochemical staining of PD-L1 in bladder tumors and its relationship to tumor histologic type, grade, and overall survival has been incompletely analyzed. Slides from 165 cystectomy specimens were reviewed for tumor type, grade of urothelial carcinoma, pathologic stage, and overall survival. A tissue microarray (TMA) using four 0.6 mm cores from each case was constructed. Immunohistochemistry was performed on the TMA using a variety of new PD-L1 antibodies and platforms now widely available. For each case, the percent of tumor cells positive for PD-L1 and the percent of positive immune cells were scored. The overall number of bladder cancers positive for PD-L1 depended on the antibody/platform combination used and the threshold for considering a tumor “PD-L1–positive.” Squamous cell carcinomas (SCCs) of the bladder demonstrated PD-L1 positivity more frequently than urothelial cell carcinomas (UCCs). High-grade UCCs were positive for PD-L1 on tumor cells more frequently than low-grade UCCs. There was no difference in survival between PD-L1–positive and PD-L1–negative bladder cancers in our study. Further studies should consider examining the predictive significance of PD-L1 IHC in bladder cancers.
•Bladder cancers are positive for PD-L1 in tumor cells and immune cells.•Squamous carcinomas are PD-L1–positive more often than urothelial carcinomas.•High-grade urothelial cancers are PD-L1–positive more often than low-grade tumors.•PD-L1–positive and PD-L1–negative bladder cancers show no difference in survival.
Details
- Title: Subtitle
- PD-L1 expression in tumor cells and the immunologic milieu of bladder carcinomas: a pathologic review of 165 cases
- Creators
- Jonathan J Davick - University of VirginiaHenry F Frierson - University of VirginiaMark Smolkin - University of VirginiaAlejandro A Gru - University of Virginia
- Resource Type
- Journal article
- Publication Details
- Human pathology, Vol.81, pp.184-191
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.humpath.2018.06.028
- PMID
- 29969606
- ISSN
- 0046-8177
- eISSN
- 1532-8392
- Language
- English
- Date published
- 11/2018
- Academic Unit
- Pathology
- Record Identifier
- 9984186482602771
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