PDE6 in lamprey Petromyzon marinus: implications for the evolution of the visual effector in vertebrates

Hakim Muradov; Kimberly K Boyd; Vasily Kerov; Nikolai O Artemyev

doi:10.1021/bi700535s

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PDE6 in lamprey Petromyzon marinus: implications for the evolution of the visual effector in vertebrates

Journal article

Peer reviewed

PDE6 in lamprey Petromyzon marinus: implications for the evolution of the visual effector in vertebrates

Hakim Muradov, Kimberly K Boyd, Vasily Kerov and Nikolai O Artemyev

Biochemistry (Easton), Vol.46(35), pp.9992-10000

09/04/2007

DOI: 10.1021/bi700535s

PMID: 17685558

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Abstract

Photoreceptor rod and cone phosphodiesterases comprise the sixth family of cyclic nucleotide phosphodiesterases (PDE6). PDE6s have uniquely evolved as effector enzymes in the vertebrate phototransduction cascade. To understand the evolution of the PDE6 family, we have examined PDE6 in lamprey, an ancient vertebrate group. A single PDE6 catalytic subunit transcript was found in the sea lamprey Petromyzon marinus cDNA library. The lamprey PDE6 sequence showed a high degree of homology with mammalian PDE6 and equally distant relationships with the rod and cone enzymes. In contrast, two different PDE6 inhibitory Pgamma subunits, a cone-type Pgamma1 and a mixed cone/rod-type Pgamma2, have been identified in the lamprey retina. Immunofluorescence analysis demonstrated that Pgamma1 and Pgamma2 are expressed in the long and short photoreceptors of sea lamprey, respectively. The catalytic PDE6 subunit was present in the photoreceptors of both types and colocalized with the Pgamma subunits. Recombinant Pgamma1 and Pgamma2 potently inhibited trypsin-activated lamprey and bovine PDE6 enzymes. Our results point to a high degree of conservation of PDE6 genes during the vertebrate evolution. The apparent duplication of the Pgamma gene in the stem of vertebrate lineage may have been an essential component of the evolution of scotopic vision in early vertebrates.

Immunohistochemistry

Amino Acid Sequence

Protein Subunits

Catalytic Domain

Cyclic Nucleotide Phosphodiesterases, Type 6 - metabolism

Vertebrates - genetics

Molecular Sequence Data

Cyclic Nucleotide Phosphodiesterases, Type 6 - chemistry

Cyclic Nucleotide Phosphodiesterases, Type 6 - genetics

Petromyzon - genetics

Phylogeny

Photoreceptor Cells, Vertebrate - enzymology

Sequence Homology, Amino Acid

Sequence Alignment

Animals

Retinal Cone Photoreceptor Cells - enzymology

Cattle

Retinal Rod Photoreceptor Cells - enzymology

Binding Sites

Evolution, Molecular

Details

Title: Subtitle: PDE6 in lamprey Petromyzon marinus: implications for the evolution of the visual effector in vertebrates
Creators: Hakim Muradov - Department of Molecular Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Kimberly K Boyd
Vasily Kerov
Nikolai O Artemyev
Resource Type: Journal article
Publication Details: Biochemistry (Easton), Vol.46(35), pp.9992-10000
Publisher: United States
DOI: 10.1021/bi700535s
PMID: 17685558
ISSN: 0006-2960
eISSN: 1520-4995
Grant note: R01 EY010843 / NEI NIH HHS EY10843 / NEI NIH HHS
Language: English
Date published: 09/04/2007
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9984070715702771

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