Journal article
PE-11, a peptide derived from chromogranin B, in the rat eye
Peptides (New York, N.Y. : 1980), Vol.32(6), pp.1201-1206
2011
DOI: 10.1016/j.peptides.2011.03.011
PMID: 21439336
Abstract
► PE-11 is a peptide which is generated in vivo by proteolytic processing of chromogranin B. ► PE-11 is a constituent of capsaicin-sensitive sensory neurons in the peripheral innervation of the rat eye. ► The presence of PE-11 in the rat retina in glia is atypical for neuropeptides and unique.
The aim of the study was to investigate the presence and distribution of PE-11, a peptide derived from chromogranin B, in the rat eye. For this purpose, newborn rats were injected with a single dosage of 50
mg/kg capsaicin subcutaneously under the neck fold and after three months, particular eye tissues were dissected and the concentration of PE-11-like immunoreactivity was determined by radioimmunoassay. Furthermore, PE-11-like immunoreactivities were characterized in an extract of the rat eye by reversed phase HPLC. Then, the distribution pattern of PE-11 was investigated in the rat eye and rat trigeminal ganglion by immunofluorescence. As a result, PE-11 was present in each tissue of the rat eye and capsaicin pretreatment led to a 88.05% (±7.07) and a 64.26% (±14.17) decrease of the levels of PE-11 in the cornea and choroid/sclera, respectively, and to a complete loss in the iris/ciliary body complex. Approximately 70% of immunoreactivities detected by the PE-11 antiserum have been found to represent authentic PE-11. Sparse nerve fibers were visualized in the corneal and uveal stroma, surrounding blood vessels at the limbus, ciliary body and choroid and in association with the dilator and sphincter muscle. Furthermore, immunoreactivity was present in the corneal endothelium. In the retina and optic nerve, glia was labeled. In the rat trigeminal ganglion, PE-11-immunoreactivity was visualized in small and medium sized ganglion cells with a diameter of up to 30
μm. In conclusion, there is unequivocal evidence that PE-11 is a constituent of capsaicin-sensitive sensory neurons innervating the rat eye and the distribution pattern is typically peptidergic in the peripheral innervation but in the retina completely atypical for neuropeptides and unique.
Details
- Title: Subtitle
- PE-11, a peptide derived from chromogranin B, in the rat eye
- Creators
- Katrin Lorenz - Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55131 Mainz, GermanyJosef Troger - Department of Ophthalmology, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaOliver Gramlich - Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55131 Mainz, GermanyFranz Grus - Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg-University Mainz, Langenbeckstraße 1, 55131 Mainz, GermanyRosa Hattmannstorfer - Clinical Department of Restorative and Prosthetic Dentistry, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaReiner Fischer-Colbrie - Department of Pharmacology, Medical University of Innsbruck, Peter Mayrstraße 1a, 6020 Innsbruck, AustriaStephanie Joachim - Center for Vision Science, Ruhr University Eye Hospital, In der Schornau 23–25, 44892 Bochum, GermanyEduard Schmid - Department of Ophthalmology, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaBarbara Teuchner - Department of Ophthalmology, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaGertrud Haas - Department of Ophthalmology, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaNikolaos Bechrakis - Department of Ophthalmology, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, Austria
- Resource Type
- Journal article
- Publication Details
- Peptides (New York, N.Y. : 1980), Vol.32(6), pp.1201-1206
- DOI
- 10.1016/j.peptides.2011.03.011
- PMID
- 21439336
- NLM abbreviation
- Peptides
- ISSN
- 0196-9781
- eISSN
- 1873-5169
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2011
- Academic Unit
- Iowa Neuroscience Institute; Neuroscience and Pharmacology; Ophthalmology and Visual Sciences
- Record Identifier
- 9984070307502771
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