Journal article
PGC-1α (Peroxisome Proliferator-Activated Receptor γ Coactivator 1-α) Overexpression in Coronary Artery Disease Recruits NO and Hydrogen Peroxide During Flow-Mediated Dilation and Protects Against Increased Intraluminal Pressure
Hypertension (Dallas, Tex. 1979), Vol.70(1), pp.166-173
07/2017
DOI: 10.1161/HYPERTENSIONAHA.117.09289
PMCID: PMC5485836
PMID: 28533333
Abstract
Blood flow through healthy human vessels releases NO to produce vasodilation, whereas in patients with coronary artery disease (CAD), the mediator of dilation transitions to mitochondria-derived hydrogen peroxide (
H
O
). Excessive
H
O
production contributes to a proatherosclerotic vascular milieu. Loss of PGC-1α (peroxisome proliferator-activated receptor γ coactivator 1α) is implicated in the pathogenesis of CAD. We hypothesized that PGC-1α suppresses
H
O
production to reestablish NO-mediated dilation in isolated vessels from patients with CAD. Isolated human adipose arterioles were cannulated, and changes in lumen diameter in response to graded increases in flow were recorded in the presence of PEG (polyethylene glycol)-catalase (H
O
scavenger) or L-NAME (
-nitro-l-arginine methyl ester; NOS inhibitor). In contrast to the exclusively NO- or H
O
-mediated dilation seen in either non-CAD or CAD conditions, respectively, flow-mediated dilation in CAD vessels was sensitive to both L-NAME and PEG-catalase after PGC-1α upregulation using ZLN005 and α-lipoic acid. PGC-1α overexpression in CAD vessels protected against the vascular dysfunction induced by an acute increase in intraluminal pressure. In contrast, downregulation of PGC-1α in non-CAD vessels produces a CAD-like phenotype characterized by
H
O
-mediated dilation (no contribution of NO). Loss of PGC-1α may contribute to the shift toward the
H
O
-mediated dilation observed in vessels from subjects with CAD. Strategies to boost PGC-1α levels may provide a therapeutic option in patients with CAD by shifting away from
H
O
-mediated dilation, increasing NO bioavailability, and reducing levels of
H
O
Furthermore, increased expression of PGC-1α allows for simultaneous contributions of both NO and H
O
to flow-mediated dilation.
Details
- Title: Subtitle
- PGC-1α (Peroxisome Proliferator-Activated Receptor γ Coactivator 1-α) Overexpression in Coronary Artery Disease Recruits NO and Hydrogen Peroxide During Flow-Mediated Dilation and Protects Against Increased Intraluminal Pressure
- Creators
- Andrew O Kadlec - Medical College of WisconsinDawid S Chabowski - Medical College of WisconsinKarima Ait-Aissa - Medical College of WisconsinJoseph C Hockenberry - Medical College of WisconsinMary F Otterson - Medical College of WisconsinMatthew J Durand - Medical College of WisconsinJulie K Freed - Medical College of WisconsinAndreas M Beyer - Medical College of WisconsinDavid D Gutterman - Medical College of Wisconsin
- Resource Type
- Journal article
- Publication Details
- Hypertension (Dallas, Tex. 1979), Vol.70(1), pp.166-173
- DOI
- 10.1161/HYPERTENSIONAHA.117.09289
- PMID
- 28533333
- PMCID
- PMC5485836
- ISSN
- 0194-911X
- eISSN
- 1524-4563
- Grant note
- R01 HL113612 / NHLBI NIH HHS R01 HL133029 / NHLBI NIH HHS R01 HL135901 / NHLBI NIH HHS T32 GM080202 / NIGMS NIH HHS
- Language
- English
- Date published
- 07/2017
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984359567102771
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