Journal article
PHARMACOLOGICAL BIOCOMPATIBILITY BETWEEN INTRAVESICAL PREPARATIONS OF BCG AND INTERFERON-ALPHA 2B
The Journal of urology, Vol.158(6), pp.2311-2315
1997
DOI: 10.1016/S0022-5347(01)68241-7
PMID: 9366382
Abstract
To determine if BCG and interferon alpha-2B are mutually compatible as mixed intravesical agents for clinical bladder cancer therapy.
Mutual compatibility was assessed by measuring IFN-alpha's effect on BCG metabolic activity, growth rate, and clumping tendency and conversely by observing BCG's effect on IFN-alpha's anti-viral activity. Optical density at 600 nm. (OD600) was used to estimate the number of colony forming units of BCG in suspension during 3 hours measurements of clumping and 8 days measurements of BCG proliferation. BCG viability was evaluated using a substrate marker, MTT, which correlates with BCG density and metabolic activity. The anti-viral activity of IFN-alpha was determined in a cytopathic protection bioassay using the encephalomyocarditis virus/FS-4 cell system.
Continuous shaking of reconstituted BCG for 3 hours at 37C resulted in a marginal (11.3%) drop in OD600 which was minimally altered by inclusion of IFN-alpha at 2 million units (MU)/ml. (12.7% drop). Metabolic activity and growth rate of BCG alone or BCG with IFN-alpha were essentially identical. IFN-alpha's antiviral activity was not affected by incubation with BCG.
The inclusion of IFN-alpha into the usual BCG formulation for intravesical administration has no apparent effect on BCG's viability or tendency to form clumps in suspension. Similarly, the physical mixing of IFN-alpha with BCG does not impair its biological activity. Thus, both agents are pharmacologically compatible for future clinical studies involving combination intravesical therapy.
Details
- Title: Subtitle
- PHARMACOLOGICAL BIOCOMPATIBILITY BETWEEN INTRAVESICAL PREPARATIONS OF BCG AND INTERFERON-ALPHA 2B
- Creators
- Tracy M. Downs - Beth Israel Deaconess Medical CenterAkos Szilvasi - Beth Israel Deaconess Medical CenterMichael A. O'Donnell
- Resource Type
- Journal article
- Publication Details
- The Journal of urology, Vol.158(6), pp.2311-2315
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0022-5347(01)68241-7
- PMID
- 9366382
- ISSN
- 0022-5347
- eISSN
- 1527-3792
- Language
- English
- Date published
- 1997
- Academic Unit
- Urology
- Record Identifier
- 9984319980602771
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