Journal article
PIM3 kinase promotes tumor metastasis in hepatoblastoma by upregulating cell surface expression of chemokine receptor cxcr4
Clinical & experimental metastasis, Vol.39(6), pp.899-912
12/01/2022
DOI: 10.1007/s10585-022-10186-3
PMCID: PMC9753553
PMID: 36315303
Abstract
Patients presenting with metastatic hepatoblastoma have limited treatment options and survival rates as low as 25%. We previously demonstrated that Proviral Integration site in Maloney murine leukemia virus 3 (PIM3) kinase promotes tumorigenesis and cancer cell stemness in hepatoblastoma. In this study, we assessed the role of PIM3 kinase in promoting hepatoblastoma metastasis. We utilized a tail vein injection model of metastasis to evaluate the effect of CRISPR/Cas9-mediated PIM3 knockout, stable overexpression of PIM3, and pharmacologic PIM inhibition on the formation of lung metastasis. In vivo studies revealed PIM3 knockout impaired the formation of lung metastasis: 5 out of 6 mice injected with wild type hepatoblastoma cells developed lung metastasis while none of the 7 mice injected with PIM3 knockout hepatoblastoma cells developed lung metastasis. PIM3 overexpression in hepatoblastoma increased the pulmonary metastatic burden in mice and mechanistically, upregulated the phosphorylation and cell surface expression of CXCR4, a key receptor in the progression of cancer cell metastasis. CXCR4 blockade with AMD3100 decreased the metastatic phenotype of PIM3 overexpressing cells, indicating that CXCR4 contributed to PIM3's promotion of hepatoblastoma metastasis. Clinically, PIM3 expression correlated positively with CXCR4 expression in primary hepatoblastoma tissues. In conclusion, we have shown PIM3 kinase promotes the metastatic phenotype of hepatoblastoma cells through upregulation of CXCR4 cell surface expression and these findings suggest that targeting PIM3 kinase may provide a novel therapeutic strategy for metastatic hepatoblastoma.
Details
- Title: Subtitle
- PIM3 kinase promotes tumor metastasis in hepatoblastoma by upregulating cell surface expression of chemokine receptor cxcr4
- Creators
- Raoud Marayati - University of Alabama at BirminghamJanet Julson - University of Alabama at BirminghamLaura V Bownes - University of Alabama at BirminghamColin H Quinn - University of Alabama at BirminghamLaura L Stafman - University of Alabama at BirminghamAndee M Beierle - University of Alabama at BirminghamHooper R Markert - University of Alabama at BirminghamSara C Hutchins - University of Alabama at BirminghamJerry E Stewart - University of Alabama at BirminghamDavid K Crossman - University of Alabama at BirminghamAnita B Hjelmeland - University of Alabama at BirminghamElizabeth Mroczek-Musulman - Department of Pathology, The Children's Hospital of Alabama, 35233, Birmingham, AL, USAElizabeth A Beierle - , 1600 7th Ave South Lowder Room 300, 35233, Birmingham, AL, USA. elizabeth.beierle@childrensal.org
- Resource Type
- Journal article
- Publication Details
- Clinical & experimental metastasis, Vol.39(6), pp.899-912
- DOI
- 10.1007/s10585-022-10186-3
- PMID
- 36315303
- PMCID
- PMC9753553
- NLM abbreviation
- Clin Exp Metastasis
- ISSN
- 0262-0898
- eISSN
- 1573-7276
- Grant note
- T32 CA229102 / NCI NIH HHS T32 GM008361 / NIGMS NIH HHS T32 CA091078 / NCI NIH HHS P30 AR048311 / NIAMS NIH HHS P30 CA013148 / NCI NIH HHS
- Language
- English
- Date published
- 12/01/2022
- Academic Unit
- Stead Family Department of Pediatrics; Hematology/Oncology
- Record Identifier
- 9984701553102771
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