PLUNC is a secreted product of neutrophil granules

Jennifer A Bartlett; Benjamin J Hicks; William M Nauseef; Jamie M Schlomann; Paul B McCray Jr; Shyam Ramachandran

doi:10.1189/jlb.0507302

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PLUNC is a secreted product of neutrophil granules

Journal article

Open access

Peer reviewed

PLUNC is a secreted product of neutrophil granules

Jennifer A Bartlett, Benjamin J Hicks, William M Nauseef, Jamie M Schlomann, Paul B McCray Jr and Shyam Ramachandran

Journal of leukocyte biology, Vol.83(5), pp.1201-1206

05/2008

DOI: 10.1189/jlb.0507302

PMID: 18245229

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Abstract

Airway epithelia and neutrophils are frequently recruited to release host defense factors in response to a variety of pulmonary pathogens. One abundant product of airway epithelia is palate, lung, nasal epithelium clone (PLUNC), a proposed innate immune protein expressed in submucosal glands and surface airway epithelia. In this study, we report the expression of PLUNC in human neutrophils, a previously unrecognized source of this protein. Immunoblots performed on polymorphonuclear cell (PMN) lysates and PMN subcellular fractions indicated that PLUNC was present in the specific granules of the neutrophil. Furthermore, secretion assays demonstrated that PLUNC protein was released by neutrophils upon stimulation with secretogogues, including formyl methionyl leucyl phenylalanine and the calcium ionophore A23187. Although recombinant PLUNC protein failed to exhibit antibacterial activity in our studies, its storage and secretion by a professional phagocytic cell support the hypothesis that PLUNC participates in an aspect of the inflammatory response that contributes to host defense. These studies suggest that PLUNC expression is less restricted than previously believed, and highlight new avenues of research for the study of PLUNC function.

Transfection

Glycoproteins - genetics

Recombinant Proteins - metabolism

Biomarkers, Tumor - secretion

Cell Line

Respiratory Mucosa - cytology

Electrophoresis, Polyacrylamide Gel

Humans

Cells, Cultured

Phosphoproteins - secretion

Cytoplasmic Granules - secretion

Phosphoproteins - genetics

Mammals

Glycoproteins - secretion

Animals

Glycoproteins - isolation & purification

Neutrophils - secretion

Phosphoproteins - isolation & purification

Respiratory Mucosa - secretion

Inflammation - physiopathology

Details

Title: Subtitle: PLUNC is a secreted product of neutrophil granules
Creators: Jennifer A Bartlett - Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
Benjamin J Hicks
William M Nauseef
Jamie M Schlomann
Paul B McCray Jr
Shyam Ramachandran
Resource Type: Journal article
Publication Details: Journal of leukocyte biology, Vol.83(5), pp.1201-1206
DOI: 10.1189/jlb.0507302
PMID: 18245229
ISSN: 0741-5400
eISSN: 1938-3673
Grant note: AI-34879 / NIAID NIH HHS I01 BX000513 / BLRD VA P50 HL-61234 / NHLBI NIH HHS P50 HL061234 / NHLBI NIH HHS
Language: English
Date published: 05/2008
Academic Unit: Microbiology and Immunology; Pulmonary Medicine; Infectious Diseases; Stead Family Department of Pediatrics; Internal Medicine
Record Identifier: 9984093222902771

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