Journal article
PROTEIN KINASE C PATHWAY IS INVOLVED IN REGULATING THE SECRETION OF PROSTATIC ACID PHOSPHATASE IN HUMAN PROSTATE CANCER CELLS
Cell biology international, Vol.25(11), pp.1139-1148
Received 17 January 2001; Accepted 7 June 2001
11/2001
DOI: 10.1006/cbir.2001.0794
PMID: 11913958
Abstract
The stimulated secretion of prostatic acid phosphatase (PAcP) has been known to be a hallmark of androgen action on human prostate epithelial cells for the last five decades. The molecular mechanism of androgen action on PAcP secretion, however, has remained mostly unknown. We investigated the molecular mechanism that promotes PAcP secretion in LNCaP human prostate carcinoma cells which express PAcP and are androgen-responsive. Treatment with 12-o-tetradecanoyl phorbol-13-acetate (TPA), a protein kinase C (PKC) activator, resulted in an increased secretion of PAcP in a dose- and time-dependent fashion. 4Alpha-phorbol, a biologically inactive isomer of TPA, had no effect. This TPA stimulation of PAcP secretion was observed 2 h after exposure, while TPA did not have a significant effect on the mRNA level even with a 6 h treatment. A23187 calcium ionophore, known to mobilize cellular calcium which is a co-factor of PKC, also activated PAcP secretion. This TPA stimulation of PAcP secretion was more potent than the conventional stimulating agent 5alpha-dihydrotestosterone (DHT) at the same concentration of 50 nM. Furthermore, the action of TPA and DHT on PAcP secretion was blocked by five different PKC inhibitors. Results also showed that DHT, as well as TPA, could rapidly modulate PKC activity. Therefore, PKC can regulate PAcP secretion, and may also be involved in DHT action on PAcP secretion.
Details
- Title: Subtitle
- PROTEIN KINASE C PATHWAY IS INVOLVED IN REGULATING THE SECRETION OF PROSTATIC ACID PHOSPHATASE IN HUMAN PROSTATE CANCER CELLS
- Creators
- Ming-Fong Lin - Department of Biochemistry/Molecular Biology, Section of Urologic Surgery, University of Nebraska Medical Center, and Omaha VA Medical Center, Omaha, NE 68198, U.S.AXiu-Qing Zhang - Department of Biochemistry/Molecular Biology, Section of Urologic Surgery, University of Nebraska Medical Center, and Omaha VA Medical Center, Omaha, NE 68198, U.S.AJeanenne Dean - Department of Biochemistry/Molecular Biology, Section of Urologic Surgery, University of Nebraska Medical Center, and Omaha VA Medical Center, Omaha, NE 68198, U.S.AFen-Fen Lin - Department of Biochemistry/Molecular Biology, Section of Urologic Surgery, University of Nebraska Medical Center, and Omaha VA Medical Center, Omaha, NE 68198, U.S.A
- Resource Type
- Journal article
- Publication Details
- Cell biology international, Vol.25(11), pp.1139-1148
- Edition
- Received 17 January 2001; Accepted 7 June 2001
- Publisher
- Blackwell Publishing Ltd
- DOI
- 10.1006/cbir.2001.0794
- PMID
- 11913958
- ISSN
- 1065-6995
- eISSN
- 1095-8355
- Number of pages
- 10
- Language
- English
- Date published
- 11/2001
- Academic Unit
- General Internal Medicine; Internal Medicine
- Record Identifier
- 9984094563502771
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