Journal article
Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex
eLife, Vol.10, 67999
07/02/2021
DOI: 10.7554/eLife.67999
PMCID: PMC8282337
PMID: 34212861
Abstract
The Drosophila polarity protein Crumbs is essential for the establishment and growth of the apical domain in epithelial cells. The protein Yurt limits the ability of Crumbs to promote apical membrane growth, thereby defining proper apical/lateral membrane ratio that is crucial for forming and maintaining complex epithelial structures such as tubes or acini. Here, we show that Yurt also increases Myosin-dependent cortical tension downstream of Crumbs. Yurt overexpression thus induces apical constriction in epithelial cells. The kinase aPKC phosphorylates Yurt, thereby dislodging the latter from the apical domain and releasing apical tension. In contrast, the kinase Pak1 promotes Yurt dephosphorylation through activation of the phosphatase PP2A. The Pak1-PP2A module thus opposes aPKC function and supports Yurt-induced apical constriction. Hence, the complex interplay between Yurt, aPKC, Pak1, and PP2A contributes to the functional plasticity of Crumbs. Overall, our data increase our understanding of how proteins sustaining epithelial cell polarization and Myosin-dependent cell contractility interact with one another to control epithelial tissue architecture.
Details
- Title: Subtitle
- Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex
- Creators
- Cornelia Biehler - Université LavalKatheryn E. Rothenberg - University of TorontoAlexandra Jette - Université LavalHelori-Mael Gaude - Université LavalRodrigo Fernandez-Gonzalez - University of TorontoPatrick Laprise - Université Laval
- Resource Type
- Journal article
- Publication Details
- eLife, Vol.10, 67999
- Publisher
- Elife Sciences Publications Ltd
- DOI
- 10.7554/eLife.67999
- PMID
- 34212861
- PMCID
- PMC8282337
- ISSN
- 2050-084X
- eISSN
- 2050-084X
- Number of pages
- 26
- Grant note
- FRQ-S MOP-142236; MOP-156279 / Canadian Institute of Health Research (CIHR); Canadian Institutes of Health Research (CIHR)
- Language
- English
- Date published
- 07/02/2021
- Academic Unit
- Biology
- Record Identifier
- 9984696710002771
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