Parabrachial Foxp2-expressing neurons are necessary for sustaining core body temperature in the cold

This work was supported by the National Institute of Neurological Disorders and Stroke (NS099425, NS130038) and the University of Iowa Aging Mind and Brain Initiative (AMBI). We thank Michael Grady for designing the thermal gradient temperature preference apparatus and Patrick Grady for assisting with the design of the algorithm for counting immunoreactive nuclei. We thank Richard Palmiter for sharing Foxp2-IRES-Cre mice. Lmx1b-2A-Cre and Nps-2A-Cre mice were generated at the University of Iowa Genome Editing Core Facility, directed by William Paradee, PhD and supported in part by grants from the NIH and from the Roy J. and Lucille A. Carver College of Medicine. We wish to thank Norma Sinclair, Patricia Yarolem, Joanne Schwarting and Rongbin Guan for their technical expertise in generating transgenic mice. For providing the Lmx1b antibody, we would like to thank Carmen Birchmeier. For assistance with the Promethion system, we would like to thank the University of Iowa Metabolic Phenotyping Core and Eric Weatherford. For assisting with the EEG/EMG recordings, we would also like to thank the Neural Circuits and Behavior Core in the Iowa Neuroscience Institute, which is supported in part by the Roy J. Carver Charitable Trust. For assisting with mouse EEG setup, we would like to thank Rui Li, PhD and Benton Purnell, PhD. For sharing E-mitters, we would like to thank George Richerson, MD PhD. For helping with stereological design, we would like to thank Hanna Stevens, MD PhD, Ben Elser PhD, and Robert Taylor. Grant sponsor: NIH (NINDS) K08 NS099425 (JCG), NIH (NINDS) R01 NS130038 (JCG).