Journal article
Parabrachial-insular stimulation does not wake mice
Journal of neurophysiology, Vol.129(2), pp.347-355
02/2023
DOI: 10.1152/jn.00318.2022
PMCID: PMC9886350
PMID: 36542422
Abstract
The parabrachial nucleus (PB) in the upper brainstem receives interoceptive information and sends a massive output projection directly to the cerebral cortex. Its glutamatergic axons primarily target the mid-insular cortex, and we have proposed that this PB-insular projection promotes arousal. Here, we test whether stimulating this projection causes wakefulness. We combined optogenetics and video-electroencephalography (vEEG) in mice to test this hypothesis by stimulating PB axons in the insular cortex. Stimulating this projection did not alter the cortical EEG or awaken mice. Also, despite a tendency toward aversion, PB-insular stimulation did not significantly alter Real-Time Place Preference (RTPP). These results are not consistent with the hypothesis that the direct PB-insular projection is part of the ascending arousal system.
Details
- Title: Subtitle
- Parabrachial-insular stimulation does not wake mice
- Creators
- Fillan S Grady - University of IowaShantelle A Graff - Department of Neurology, University of Iowa, Iowa City, IA, United StatesJon M Resch - University of IowaJoel C Geerling - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of neurophysiology, Vol.129(2), pp.347-355
- DOI
- 10.1152/jn.00318.2022
- PMID
- 36542422
- PMCID
- PMC9886350
- ISSN
- 0022-3077
- eISSN
- 1522-1598
- Grant note
- NS099425 / HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS)
- Language
- English
- Electronic publication date
- 12/21/2022
- Date published
- 02/2023
- Academic Unit
- Neurology; Iowa Neuroscience Institute; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology
- Record Identifier
- 9984339458302771
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