Paradoxical effects of galloyl motifs in the interactions of proanthocyanidins with collagen-rich dentin

Yvette Alania; Bin Zhou; Mariana Reis; Ariene A Leme-Kraus; James B McAlpine; Shao-Nong Chen; Guido F Pauli; Ana K Bedran-Russo

doi:10.1002/jbm.a.37276

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Paradoxical effects of galloyl motifs in the interactions of proanthocyanidins with collagen-rich dentin

Journal article

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Paradoxical effects of galloyl motifs in the interactions of proanthocyanidins with collagen-rich dentin

Yvette Alania, Bin Zhou, Mariana Reis, Ariene A Leme-Kraus, James B McAlpine, Shao-Nong Chen, Guido F Pauli and Ana K Bedran-Russo

Journal of biomedical materials research. Part A, Vol.110(1), pp.196-203

01/2022

DOI: 10.1002/jbm.a.37276

PMCID: PMC8900943

PMID: 34309176

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https://www.ncbi.nlm.nih.gov/pmc/articles/8900943View

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Abstract

Plant-derived proanthocyanidins (PACs) mediate physicochemical modifications to the dentin extracellular matrix (ECM). The structure-activity relationships of PACs remain largely unknown, mostly due to the varied complex composition of crude extracts, as well as the challenges of purification and mechanistic assessment. To assess the role of galloylated PACs as significant contributors to high yet unstable biomodification activity to the dentin ECM, we removed the galloyl moieties (de-galloylation) via enzymatic hydrolysis from three galloyl-rich PAC-containing extracts (Camellia sinensis, Vitis vinifera, and Hamamelis virginiana). The biomechanical and biological properties of dentin were assessed upon treatment with these extracts vs. their de-galloylated counterparts. An increase in the complex modulus of the dentin matrix was found with all extracts, however, the crude extract was significantly higher when compared to the de-galloylated version. Exhibiting the highest content of galloylated PACs among the investigated plants, Camellia sinensis crude extract also exhibited the biggest relapse in mechanical properties after one-month incubation. De-galloylation did not modify the damping capacity of dentin ECM. Moreover, PAC-mediated protection against proteolytic degradation was unaffected by de-galloylation. The de-galloylation experiments confirmed that gallic acid in galloylated rich-PAC extracts drive stronger yet significantly less sustained mechanical effects in dentin ECM.

Collagen - analysis

Dentin - chemistry

Extracellular Matrix

Proanthocyanidins - analysis

Proanthocyanidins - chemistry

Proanthocyanidins - pharmacology

Details

Title: Subtitle: Paradoxical effects of galloyl motifs in the interactions of proanthocyanidins with collagen-rich dentin
Creators: Yvette Alania - University of Illinois Chicago
Bin Zhou - University of Illinois Chicago
Mariana Reis - Marquette University
Ariene A Leme-Kraus - University of Illinois Chicago
James B McAlpine - University of Illinois Chicago
Shao-Nong Chen - University of Illinois Chicago
Guido F Pauli - University of Illinois Chicago
Ana K Bedran-Russo - Marquette University
Resource Type: Journal article
Publication Details: Journal of biomedical materials research. Part A, Vol.110(1), pp.196-203
DOI: 10.1002/jbm.a.37276
PMID: 34309176
PMCID: PMC8900943
NLM abbreviation: J Biomed Mater Res A
ISSN: 1549-3296
eISSN: 1552-4965
Grant note: R01 DE021040 / NIDCR NIH HHS R01 DE028194 / NIDCR NIH HHS R56 DE021040 / NIDCR NIH HHS
Language: English
Date published: 01/2022
Academic Unit: Operative Dentistry
Record Identifier: 9984367737702771

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