Parathyroid hormone rapidly stimulates hyaluronan synthesis by periosteal osteoblasts in the tibial diaphysis of the growing rat

Ronald J Midura; Xiaowei Su; Jose A Morcuende; Markku Tammi; Raija Tammi

doi:10.1074/jbc.M307567200

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Parathyroid hormone rapidly stimulates hyaluronan synthesis by periosteal osteoblasts in the tibial diaphysis of the growing rat

Journal article

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Parathyroid hormone rapidly stimulates hyaluronan synthesis by periosteal osteoblasts in the tibial diaphysis of the growing rat

Ronald J Midura, Xiaowei Su, Jose A Morcuende, Markku Tammi and Raija Tammi

The Journal of biological chemistry, Vol.278(51), pp.51462-51468

12/19/2003

DOI: 10.1074/jbc.M307567200

PMID: 14514685

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Abstract

Short term treatment (3-24 h) with parathyroid hormone (PTH) stimulated the synthesis and accumulation of hyaluronan (HyA) in explant cultures of tibial diaphyses from young rats. PTH increased the overall HyA content of periosteum 5-fold, with the basal cambium layer exhibiting the greatest enhancement ( approximately 8-fold). PTH increased the HyA content of cortical bone by 2-fold while not affecting the HyA content of bone marrow. PTH treatment greatly enhanced HyA staining throughout all layers of the periosteum, although its most dramatic effect occurred in the basal cambium layer. Here, unlike in the control tissue sections, nearly all cambium-lining osteoblasts stained intensely positive for HyA. PTH treatment enhanced the HyA staining of osteocytes in cortical bone tissue sections to the extent that the lacunocanalicular system became visualized. Three significant findings were revealed in this study. First, mature periosteal osteoblasts, under natural conditions, do not contain much HyA in their surrounding extracellular matrix but dramatically enhance their matrix HyA content when treated with PTH. Second, pre-osteocytes and osteocytes contain more HyA in their natural matrix than mature lining osteoblasts, and they appear to have functional PTH receptors because they responded to PTH treatment with an enhancement of HyA content. Finally, it was observed that the lining cells along the endosteal surface of the diaphysis did not stain strongly positive for HyA either naturally or when exposed to PTH treatment. This indicates that periosteal and endosteal osteoblastic cell populations exhibit metabolic differences in their extracellular matrix responses to PTH.

Animals, Newborn

Culture Techniques

Extracellular Matrix - metabolism

Rats

Male

Parathyroid Hormone - pharmacology

Periosteum - metabolism

Tritium

Diaphyses - metabolism

Tibia - drug effects

Osteoclasts - metabolism

Tibia - cytology

Osteoclasts - ultrastructure

Animals

Diaphyses - drug effects

Hyaluronic Acid - analysis

Hyaluronic Acid - biosynthesis

Staining and Labeling

Diaphyses - cytology

Osteoclasts - drug effects

Details

Title: Subtitle: Parathyroid hormone rapidly stimulates hyaluronan synthesis by periosteal osteoblasts in the tibial diaphysis of the growing rat
Creators: Ronald J Midura - Orthopaedic Research Center and the Department of Biomedical Engineering, the Lerner Research Institute of The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA. midura@bme.ri.ccf.org
Xiaowei Su
Jose A Morcuende
Markku Tammi
Raija Tammi
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.278(51), pp.51462-51468
DOI: 10.1074/jbc.M307567200
PMID: 14514685
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: AR-45171 / NIAMS NIH HHS
Language: English
Date published: 12/19/2003
Academic Unit: Stead Family Department of Pediatrics; Orthopedics and Rehabilitation
Record Identifier: 9984040460802771

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