Parental High-Fat Diet Promotes Inflammatory and Senescence-Related Changes in Prostate

Kulbhushan Tikoo; Ajit Vikram; Shweta Shrivastava; Gopabandhu Jena; Heta Shah; Richa Chhabra

doi:10.1155/2017/4962950

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Parental High-Fat Diet Promotes Inflammatory and Senescence-Related Changes in Prostate

Journal article

Open access

Peer reviewed

Parental High-Fat Diet Promotes Inflammatory and Senescence-Related Changes in Prostate

Kulbhushan Tikoo, Ajit Vikram, Shweta Shrivastava, Gopabandhu Jena, Heta Shah and Richa Chhabra

Oxidative medicine and cellular longevity, Vol.2017, pp.4962950-13

2017

DOI: 10.1155/2017/4962950

PMCID: PMC5316447

PMID: 28261375

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Abstract

. Obesity and dietary habits are associated with increased incidences of aging-related prostatic diseases. The present study was aimed to investigate transgenerational effects of chronic high-fat diet (HFD) feeding on inflammation and senescence-related changes in prostate. . Sprague-Dawley rats were kept on either normal or HFD one. Senescence-associated -galactosidase (SA -gal) activity, inflammation, and cellular proliferation were determined in the prostate. . Increased SA -gal activity, expression of p53, and cell proliferation marker PCNA were observed in ventral prostate of HFD-fed rats. Immunostaining for p53 and PCNA revealed that the p53 immunopositive cells were primarily in stroma while PCNA immunopositive cells were epithelial cells. An increase in expression of cycloxygenase-2 (COX-2) and phosphorylation of nuclear factor-kappa B (NF-kB) was observed in prostate of weaning pups HFD-fed parents. However, in adult pups, irrespective of dietary habit, a significant increase in the expression of COX-2, PCNA, phosphorylation of NF-kB, infiltration of inflammatory cells, and SA -gal activity was observed. Present investigation reports that HFD feeding promotes accumulation of p53 expressing cells, proliferation of epithelial cells, and senescence-related changes in prostate. Further, parental HFD-feeding upholds inflammatory, proliferative, and senescence-related changes in prostate of pups.

Immunohistochemistry

Inflammation - pathology

Immunoprecipitation

Insulin Resistance

Diet, High-Fat - adverse effects

Rats

Immunoblotting

Male

Inflammation - etiology

Rats, Sprague-Dawley

Pregnancy

Prostate - pathology

Animals

Cellular Senescence

Female

Obesity - etiology

Prenatal Exposure Delayed Effects - pathology

Disease Models, Animal

Details

Title: Subtitle: Parental High-Fat Diet Promotes Inflammatory and Senescence-Related Changes in Prostate
Creators: Kulbhushan Tikoo - Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India
Ajit Vikram - Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India
Shweta Shrivastava - Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India
Gopabandhu Jena - Facility for Risk Assessment and Intervention Studies, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India
Heta Shah - Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India
Richa Chhabra - Laboratory of Epigenetics and Diseases, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab 160 062, India
Resource Type: Journal article
Publication Details: Oxidative medicine and cellular longevity, Vol.2017, pp.4962950-13
DOI: 10.1155/2017/4962950
PMID: 28261375
PMCID: PMC5316447
NLM abbreviation: Oxid Med Cell Longev
ISSN: 1942-0900
eISSN: 1942-0994
Grant note: DOI: 10.13039/501100001407, name: Department of Biotechnology , Ministry of Science and Technology
Language: English
Date published: 2017
Academic Unit: Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
Record Identifier: 9984094517302771

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