Partitioning of perfluorooctanoate into phosphatidylcholine bilayers is chain length-independent

Wei Xie; Geoffrey D Bothun; Hans-Joachim Lehmler

doi:10.1016/j.chemphyslip.2010.01.003

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Partitioning of perfluorooctanoate into phosphatidylcholine bilayers is chain length-independent

Journal article

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Partitioning of perfluorooctanoate into phosphatidylcholine bilayers is chain length-independent

Wei Xie, Geoffrey D Bothun and Hans-Joachim Lehmler

Chemistry and physics of lipids, Vol.163(3), pp.300-308

2010

DOI: 10.1016/j.chemphyslip.2010.01.003

PMCID: PMC2841784

PMID: 20096277

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Abstract

The chain length dependence of the interaction of PFOA, a persistent environmental contaminant, with dimyristoyl- (DMPC), dipalmitoyl- (DPPC) and distearoylphosphatidylcholine (DSPC) was investigated using steady-state fluorescence anisotropy spectroscopy, differential scanning calorimetry (DSC) and dynamic light scattering (DLS). PFOA caused a linear depression of the main phase transition temperature T m while increasing the width of the phase transition of all three phosphatidylcholines. Although PFOA's effect on T m and the transition width decreased in the order DMPC > DPPC > DSPC, its relative effect on the phase behavior was largely independent of the phosphatidylcholine. PFOA caused swelling of DMPC but not DPPC and DSPC liposomes at 37 °C in the DLS experiments, which suggests that PFOA partitions more readily into bilayers in the fluid phase. These findings suggest that PFOA's effect on the phase behavior of phosphatidylcholines depends on the cooperativity and state (i.e., gel versus liquid phase) of the membrane. DLS experiments are also consistent with partial liposome solubilization at PFOA/lipid molar ratios > 1, which suggests the formation of mixed PFOA–lipid micelles.

Fluorinated surfactants

Model membranes

Partition coefficients

Perfluorooctanoate

Details

Title: Subtitle: Partitioning of perfluorooctanoate into phosphatidylcholine bilayers is chain length-independent
Creators: Wei Xie - Department of Occupational and Environmental Health, University of Iowa, College of Public Health, 100 Oakdale Campus #221 IREH, Iowa City, IA 52242-5000, USA
Geoffrey D Bothun - Department of Chemical Engineering, University of Rhode Island, Kingston, RI 02881, USA
Hans-Joachim Lehmler - Department of Occupational and Environmental Health, University of Iowa, College of Public Health, 100 Oakdale Campus #221 IREH, Iowa City, IA 52242-5000, USA
Resource Type: Journal article
Publication Details: Chemistry and physics of lipids, Vol.163(3), pp.300-308
DOI: 10.1016/j.chemphyslip.2010.01.003
PMID: 20096277
PMCID: PMC2841784
NLM abbreviation: Chem Phys Lipids
ISSN: 0009-3084
eISSN: 1873-2941
Publisher: Elsevier Ireland Ltd
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health; DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences
Language: English
Date published: 2010
Academic Unit: Occupational and Environmental Health; Iowa Neuroscience Institute; Iowa Superfund Research Program
Record Identifier: 9984001079502771

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