Journal article
Passive airborne dust sampling with the electrostatic dustfall collector: optimization of storage and extraction procedures for endotoxin and glucan measurement
Annals of Occupational Hygiene, Vol.54(6), pp.651-658
08/01/2010
DOI: 10.1093/annhyg/meq026
Abstract
We recently introduced a passive dust sampling method for airborne endotoxin and glucan exposure assessment-the electrostatic dustfall collector (EDC). In this study, we assessed the effects of different storage and extraction procedures on measured endotoxin and glucan levels, using 12 parallel EDC samples from 10 low exposed indoor environments. Additionally, we compared 2- and 4-week sampling with the prospect of reaching higher dust yields. Endotoxin concentrations were highest after extraction with pyrogen-free water (pf water) + Tween. Phosphate-buffered saline (PBS)-Tween yielded significantly (44%) lower levels, and practically no endotoxin was detected after extraction in pf water without Tween. Glucan levels were highest after extraction in PBS-Tween at 120 degrees C, whereas extracts made in NaOH at room temperature or 120 degrees C were completely negative. Direct extraction from the EDC cloth or sequential extraction after a preceding endotoxin extraction yielded comparable glucan levels. Sample storage at different temperatures before extraction did not affect endotoxin and glucan concentrations. Doubling the sampling duration yielded similar endotoxin and only 50% higher glucan levels. In conclusion, of the tested variables, the extraction medium was the predominant factor affecting endotoxin and glucan yields.
Details
- Title: Subtitle
- Passive airborne dust sampling with the electrostatic dustfall collector: optimization of storage and extraction procedures for endotoxin and glucan measurement
- Creators
- Dick J.J. HeederikPeter S. Thorne - University of IowaInge M. WoutersIlka NossGert DoekesIngrid Sander
- Resource Type
- Journal article
- Publication Details
- Annals of Occupational Hygiene, Vol.54(6), pp.651-658
- DOI
- 10.1093/annhyg/meq026
- ISSN
- 1475-3162
- Copyright
- © The Author 2010. Published by Oxford University Press on behalf of the British Occupational Hygiene Society
- Language
- English
- Date published
- 08/01/2010
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health
- Record Identifier
- 9983557435102771
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